Martínez A, Martínez J, Sanz M L, Bartolomé B, Palacios R
Ifidesa-Aristegui, Research and Development Department, Bilbao, Spain.
Allergy. 1994 Sep;49(8):664-7. doi: 10.1111/j.1398-9995.1994.tb00137.x.
Four different commercially available raw materials of dog epithelia (dander, hair and skin scraping, skin scraping, and whole skin) were compared by means of biochemical methods such as SDS-PAGE and chromatography with the SMART System, immunochemical methods such as RAST and SDS-PAGE immunoblotting, and cutaneous tests. Dander extract clearly exhibited the highest IgE binding and in vivo allergenic activity. The dog major allergens Can fI and the 19-kDa protein were adequately detected only in dander extract. Thus, it is concluded that dander is the most suitable epithelial source for preparing dog allergenic extracts.
