Spitzauer S, Schweiger C, Anrather J, Ebner C, Scheiner O, Kraft D, Rumpold H
Institute of Clinical Chemistry, University of Vienna, Austria.
Int Arch Allergy Immunol. 1993;100(1):60-7. doi: 10.1159/000236389.
Sera from 75 patients with clinical type I allergy against dogs were investigated by means of immunoblotting using extracts prepared from dog hair/dander (CAN XI D) and saliva. In addition, selected sera were tested on extracts made of hair, skin, salivary glands (parotis and submandibularis), serum and liver. A 69-kD IgE-binding protein was identified in all extracts tested with an incidence of approximately 40% and shown to be dog albumin by means of inhibition experiments. In 96% of patients' sera IgE antibodies reactive with a 19-kD and/or a 23-kD protein of the hair/dander extract (CAN XI D) were observed. IgE binding to a 23-kD band was also detected in the hair and saliva extracts, but not in skin, salivary gland, serum and liver extracts. A 19-kD IgE-binding protein was strongly expressed in skin and to a lesser degree in saliva, but not in hair, serum and liver. Preincubation of patients sera with the hair extract and subsequent probing with the hair/dander extract (CAN XI D) inhibited IgE binding to the 23 kD protein whereas preincubation with the skin extract abolished IgE binding to the 19-kD protein. Using the hair/dander extract as inhibitor, IgE binding to the 19- and 23-kD proteins of saliva was abrogated. Thus it is concluded that the 23-kD protein is preferentially expressed in hair and saliva whereas the 19-kD protein is found in saliva and skin. Furthermore these two proteins are likely to represent immunologically independent major allergens.
采用从狗毛/皮屑（CAN XI D）和唾液制备的提取物，通过免疫印迹法对75例临床I型犬过敏患者的血清进行了研究。此外，还选用了部分血清，检测其对毛发、皮肤、唾液腺（腮腺和下颌下腺）、血清和肝脏提取物的反应。在所有测试提取物中均鉴定出一种69-kD的IgE结合蛋白，其出现率约为40%，通过抑制实验证明该蛋白为狗白蛋白。在96%的患者血清中观察到与毛发/皮屑提取物（CAN XI D）的19-kD和/或23-kD蛋白反应的IgE抗体。在毛发和唾液提取物中也检测到IgE与23-kD条带的结合，但在皮肤、唾液腺、血清和肝脏提取物中未检测到。一种19-kD的IgE结合蛋白在皮肤中强烈表达，在唾液中表达程度较低，但在毛发、血清和肝脏中不表达。患者血清先用毛发提取物预孵育，随后用毛发/皮屑提取物（CAN XI D）进行检测，可抑制IgE与23 kD蛋白的结合，而先用皮肤提取物预孵育则可消除IgE与19-kD蛋白的结合。以毛发/皮屑提取物作为抑制剂，可消除IgE与唾液中19-kD和23-kD蛋白的结合。因此得出结论，23-kD蛋白优先在毛发和唾液中表达，而19-kD蛋白存在于唾液和皮肤中。此外，这两种蛋白可能代表免疫上独立的主要过敏原。
