一种与沙门氏菌噬菌体Felix O1极为相似的致病性大肠杆菌O157:H7噬菌体的基因组和蛋白质组。
The genome and proteome of a virulent Escherichia coli O157:H7 bacteriophage closely resembling Salmonella phage Felix O1.
作者信息
Villegas Andre, She Yi-Min, Kropinski Andrew M, Lingohr Erika J, Mazzocco Amanda, Ojha Shivani, Waddell Thomas E, Ackermann Hans-Wolfgang, Moyles Dianne M, Ahmed Rafiq, Johnson Roger P
机构信息
Public Health Agency of Canada, Laboratory for Foodborne Zoonoses, 110 Stone Road West, Guelph, ON, N1G 3W4, Canada.
出版信息
Virol J. 2009 Apr 20;6:41. doi: 10.1186/1743-422X-6-41.
Based upon whole genome and proteome analysis, Escherichia coli O157:H7-specific bacteriophage (phage) wV8 belongs to the new myoviral genus, "the Felix O1-like viruses" along with Salmonella phage Felix O1 and Erwinia amylovora phage phiEa21-4. The genome characteristics of phage wV8 (size 88.49 kb, mol%G+C 38.9, 138 ORFs, 23 tRNAs) are very similar to those of phage Felix O1 (86.16 kb, 39.0 mol%G+C, 131 ORFs and 22 tRNAs) and, indeed most of the proteins have their closest homologs within Felix O1. Approximately one-half of the Escherichia coli O157:H7 mutants resistant to phage wV8 still serotype as O157:H7 indicating that this phage may recognize, like coliphage T4, two different surface receptors: lipopolysaccharide and, perhaps, an outer membrane protein.
基于全基因组和蛋白质组分析，大肠杆菌O157:H7特异性噬菌体（噬菌体）wV8与沙门氏菌噬菌体Felix O1和梨火疫病菌噬菌体phiEa21-4同属于新的肌病毒属“类Felix O1病毒”。噬菌体wV8的基因组特征（大小88.49 kb，G+C含量38.9 mol%，138个开放阅读框，23个转运RNA）与噬菌体Felix O1（86.16 kb，39.0 mol%G+C，131个开放阅读框和22个转运RNA）非常相似，实际上大多数蛋白质在Felix O1中有最相近的同源物。约一半对噬菌体wV8具有抗性的大肠杆菌O157:H7突变体仍为O157:H7血清型，这表明该噬菌体可能像大肠杆菌噬菌体T4一样识别两种不同的表面受体：脂多糖，或许还有一种外膜蛋白。
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