噬菌体菲力克斯 O1 的全基因组序列
Complete genomic sequence of bacteriophage felix o1.
机构信息
Mailstop G29, Centers for Disease Control and Prevention; 1600 Clifton Road, Atlanta, GA 30329, USA.
Smithsonian National Institution, National Museum in Natural History, MRC 534, Washington, DC 20560, USA.
出版信息
Viruses. 2010 Mar;2(3):710-730. doi: 10.3390/v2030710. Epub 2010 Mar 9.
Bacteriophage O1 is a Myoviridae A1 group member used historically for identifying Salmonella. Sequencing revealed a single, linear, 86,155-base-pair genome with 39% average G+C content, 131 open reading frames, and 22 tRNAs. Closest protein homologs occur in Erwinia amylovora phage φEa21-4 and Escherichia coli phage wV8. Proteomic analysis indentified structural proteins: Gp23, Gp36 (major tail protein), Gp49, Gp53, Gp54, Gp55, Gp57, Gp58 (major capsid protein), Gp59, Gp63, Gp64, Gp67, Gp68, Gp69, Gp73, Gp74 and Gp77 (tail fiber). Based on phage-host codon differences, 7 tRNAs could affect translation rate during infection. Introns, holin-lysin cassettes, bacterial toxin homologs and host RNA polymerase-modifying genes were absent.
噬菌 O1 是一种肌尾噬菌体科 A1 群成员，历史上用于鉴定沙门氏菌。测序揭示了一个单一的、线性的、86155 碱基对的基因组，平均 GC 含量为 39%，有 131 个开放阅读框和 22 个 tRNA。最接近的蛋白质同源物存在于梨火疫病菌噬菌体 φEa21-4 和大肠杆菌噬菌体 wV8 中。蛋白质组学分析鉴定了结构蛋白：Gp23、Gp36（主要尾部蛋白）、Gp49、Gp53、Gp54、Gp55、Gp57、Gp58（主要衣壳蛋白）、Gp59、Gp63、Gp64、Gp67、Gp68、Gp69、Gp73、Gp74 和 Gp77（尾部纤维）。基于噬菌体-宿主密码子差异，7 个 tRNA 可能会影响感染过程中的翻译速度。内含子、溶菌酶盒、细菌毒素同源物和宿主 RNA 聚合酶修饰基因缺失。
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