Iltis J P, Daniels S B
Infect Immun. 1977 May;16(2):701-5. doi: 10.1128/iai.16.2.701-705.1977.
A method for purification of marble spleen disease virus (MSDV) from the spleens of infected turkeys and pheasants is described. It combines chloroform or fluorocarbon extraction with subsequent purification by centrifugation on a cushion of cesium chloride (CsCl). Further purification of MSDV was accomplished with a CsCl equilibrium density gradient. A viral buoyant density of approximately 1.32 to 1.33 g/cm3 was determined. Negative-stain electron microscopy revealed that virus isopycnically banded by CsCl gradient had non-enveloped icosahedral capsids composed of 252 capsomeres. The direct colorimetric diphenylamine assay indicated that MSDV has deoxyribonucleic acid as its nucleic acid. The above evidence demonstrates that MSDV is an avian adenovirus, the first recognized in the ring-necked pheasant (Phasianus colchicus L.).
本文描述了一种从感染火鸡和雉鸡脾脏中纯化大理石脾病病毒（MSDV）的方法。该方法将氯仿或氟碳化合物提取与随后在氯化铯（CsCl）垫层上进行离心纯化相结合。通过CsCl平衡密度梯度进一步纯化MSDV。测定病毒的浮力密度约为1.32至1.33 g/cm³。负染电子显微镜显示，通过CsCl梯度等密度带的病毒具有由252个壳粒组成的无包膜二十面体衣壳。直接比色二苯胺测定表明，MSDV以脱氧核糖核酸作为其核酸。上述证据表明，MSDV是一种禽腺病毒，是在环颈雉（Phasianus colchicus L.）中首次发现的。
