Di Pippo F, Bohn A, Congestri R, De Philippis R, Albertano P
Department of Biology, University of Rome Tor Vergata, Rome, Italy.
Biofouling. 2009;25(6):495-504. doi: 10.1080/08927010902914037.
Phototrophic biofilm samples from an Italian wastewater treatment plant were studied in microcosm experiments under varying irradiances, temperatures and flow regimes to assess the effects of environmental variables and phototrophic biomass on capsular exopolysaccharides (CPS). The results, obtained from circular dichroism spectroscopy and High Performance Liquid Chromatography, suggest that CPS have a stable spatial conformation and a complex monosaccharide composition. The total amount present was positively correlated with the biomass of cyanobacteria and diatoms, and negatively with the biovolume of green algae. The proportion of uronic acids showed the same correlation with these taxon groups, indicating a potential role of cyanobacteria and diatoms in the removal of residual nutrients and noxious cations in wastewater treatment. While overall biofilm growth was limited by low irradiance, high temperature (30 degrees C) and low flow velocity (25 l h(-1)) yielded the highest phototrophic biomass, the largest amount of CPS produced, and the highest proportion of carboxylic acids present.
在不同辐照度、温度和水流状态下，对取自意大利一家污水处理厂的光合生物膜样本进行了微观实验研究，以评估环境变量和光合生物量对荚膜胞外多糖（CPS）的影响。通过圆二色光谱法和高效液相色谱法获得的结果表明，CPS具有稳定的空间构象和复杂的单糖组成。其总量与蓝藻和硅藻的生物量呈正相关，与绿藻的生物体积呈负相关。糖醛酸的比例与这些分类群呈现相同的相关性，这表明蓝藻和硅藻在污水处理中去除残留营养物和有害阳离子方面具有潜在作用。虽然总体生物膜生长受到低辐照度的限制，但高温（30摄氏度）和低流速（25升/小时）产生了最高的光合生物量、产生的CPS量最大以及羧酸比例最高。
