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绵羊岩下窦插管术:一种从垂体采集激素分泌样本方法的开发

Petrosal sinus catheterization in sheep. Development of a method for sampling hormonal output from the pituitary.

作者信息

Aitken W M, Bass J J, Heap S W, Stuart S P, Spencer G S, Hodgkinson S C

机构信息

Ruakura Agricultural Center, Hamilton, New Zealand.

出版信息

Invest Radiol. 1991 Sep;26(9):825-8. doi: 10.1097/00004424-199109000-00009.

DOI:10.1097/00004424-199109000-00009
PMID:1938293
Abstract

A relatively nontraumatic method has been developed to catheterize the petrosal sinus (PS) of sheep, via the internal jugular vein (IJV), using a percutaneous approach monitored by fluoroscopy. Preselection of suitable animals was facilitated by injecting radiopaque material through a cannula inserted into the deep facial vein to display the venous drainage from the pituitary. Further injections, via the same cannula, were later used to assist in the maneuvering of the catheter/wire guide combination as it passed up the IJV. To confirm catheter placement, plasma samples, collected simultaneously from PS and external jugular vein (EJV), were analyzed for growth hormone (GH). GH concentrations were consistently higher in the PS samples than in those found in the EJV, and more GH pulses were seen in PS samples than in the general circulation.

摘要

已开发出一种相对无创的方法,通过颈内静脉(IJV),采用透视监测下的经皮穿刺法,对绵羊的岩下窦(PS)进行插管。通过将不透射线的材料注入插入面深部静脉的套管中,以显示垂体的静脉引流,从而便于预先挑选合适的动物。随后,通过同一套管进行进一步注射,以协助导管/导丝组合在沿颈内静脉上行时的操作。为确认导管位置,同时从岩下窦和颈外静脉(EJV)采集血浆样本,分析其中的生长激素(GH)。岩下窦样本中的生长激素浓度始终高于颈外静脉样本,且岩下窦样本中出现的生长激素脉冲比体循环中更多。

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