Irvine C H, Alexander S L
J Endocrinol. 1987 May;113(2):183-92. doi: 10.1677/joe.0.1130183.
We have described a novel technique for collecting pituitary venous effluent in the horse by placing a cannula in the intercavernous sinus close to the outlet of the pituitary veins using a venous pathway unique to equids. Cannula placement and blood collection are carried out painlessly in fully conscious, ambulatory, unstressed animals. There is no interference to hypothalamic, pituitary or target organ function. The blood collected contains readily measurable concentrations of gonadotrophin-releasing hormone, and LH concentrations which can be up to 40 times those in concurrent peripheral blood samples. Four millilitre blood samples, a quantity which permits simultaneous measurement of many hypothalamic and pituitary hormones, can be collected at 2-min intervals for several days. Intercavernous sinus blood flow can be calculated allowing secretion rates of hypothalamic and pituitary hormones to be determined for any time-period. This model is uniquely useful for investigating the normal functional characteristics of several neuroendocrine and endocrine systems.
我们描述了一种通过使用马独有的静脉通路，将套管置于靠近垂体静脉出口的海绵间窦来收集马垂体静脉流出液的新技术。在完全清醒、可自由活动且无应激的动物身上，套管放置和采血过程无痛进行。对下丘脑、垂体或靶器官功能无干扰。所采集的血液中含有易于测量浓度的促性腺激素释放激素，以及促黄体生成素浓度，其浓度可达同期外周血样本的40倍。每隔2分钟可采集4毫升血液样本，该血量允许同时测量多种下丘脑和垂体激素，可持续数天。可计算海绵间窦血流量，从而确定任何时间段下丘脑和垂体激素的分泌率。该模型对于研究多个神经内分泌和内分泌系统的正常功能特征具有独特的用途。
