钙化软骨的矿物相：其分子结构及与有机基质的界面
The mineral phase of calcified cartilage: its molecular structure and interface with the organic matrix.
作者信息
Duer Melinda J, Friscić Tomislav, Murray Rachel C, Reid David G, Wise Erica R
机构信息
Department of Chemistry, University of Cambridge, Cambridge, United Kingdom.
出版信息
Biophys J. 2009 Apr 22;96(8):3372-8. doi: 10.1016/j.bpj.2008.12.3954.
We have studied the atomic level structure of mineralized articular cartilage with heteronuclear solid-state NMR, our aims being to identify the inorganic species present at the surfaces of the mineral crystals which may interact with the surrounding organic matrix and to determine which components of the organic matrix are most closely involved with the mineral crystals. One-dimensional (1)H and (31)P and two-dimensional (1)H-(31)P heteronuclear correlation NMR experiments show that the mineral component is very similar to that in bone with regard to its surface structure. (13)C{(31)P} rotational echo double resonance experiments identify the organic molecules at the mineral surface as glycosaminoglycans, which concurs with our recent finding in bone. There is also evidence of gamma-carboxyglutamic acid residues interacting with the mineral. However, other matrix components appear more distant from the mineral compared with bone. This may be due to a larger hydration layer on the mineral crystal surfaces in calcified cartilage.
我们利用异核固态核磁共振研究了矿化关节软骨的原子水平结构，目的是确定存在于矿物晶体表面的可能与周围有机基质相互作用的无机物种，并确定有机基质的哪些成分与矿物晶体关系最为密切。一维（1）H和（31）P以及二维（1）H - （31）P异核相关核磁共振实验表明，就其表面结构而言，矿物成分与骨中的非常相似。（13）C{（31）P}旋转回波双共振实验确定矿物表面的有机分子为糖胺聚糖，这与我们最近在骨中的发现一致。也有证据表明γ-羧基谷氨酸残基与矿物相互作用。然而，与骨相比，其他基质成分似乎与矿物的距离更远。这可能是由于钙化软骨中矿物晶体表面的水化层更大。
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