Chen J, Zhang Q, McCulloch C A, Sodek J
Medical Research Council Group in Periodontal Physiology, Faculty of Dentistry, University of Toronto, Ontario, Canada.
Histochem J. 1991 Jun;23(6):281-9. doi: 10.1007/BF01045047.
Bone sialoprotein (BSP) is a prominent component of bone tissues that is expressed by differentiated osteoblastic cells. Affinity-purified antibodies to BSP were prepared and used in combination with biotin-conjugated peroxidase-labeled second antibodies to demonstrate the distribution of this protein in sections of demineralized foetal porcine tibia and calvarial bone. Staining for BSP was observed in the matrix of mineralized bone and also in the mineralized cartilage and associated cells of the epiphysis, but was not observed in the hypertrophic zone nor in any of the soft tissues including the periosteum. In comparison, SPP-1 (osteopontin) and SPARC (osteonectin), which are also major proteins in porcine bone, were observed in the cartilage as well as in the mineralized bone matrix. In addition, SPARC was also present in soft connective tissues. Although SPP-1 distribution was more restricted than SPARC, hypertrophic chondrocytes, periosteal cells and some stromal cells in the bone marrow spaces were stained in addition to osteoblastic cells. The variations in the distribution and cellular expression of BSP, SPARC and SPP-1 in bone and mineralizing cartilage indicate these proteins perform different functions in the formation and remodelling of mineralized connective tissues.
骨唾液蛋白（BSP）是骨组织的一种主要成分，由分化的成骨细胞表达。制备了针对BSP的亲和纯化抗体，并将其与生物素偶联的过氧化物酶标记二抗结合使用，以显示该蛋白在脱矿胎猪胫骨和颅骨切片中的分布。在矿化骨的基质以及骨骺的矿化软骨和相关细胞中观察到BSP染色，但在肥大区以及包括骨膜在内的任何软组织中均未观察到。相比之下，同样是猪骨主要蛋白的SPP-1（骨桥蛋白）和SPARC（骨连接蛋白）在软骨以及矿化骨基质中均有观察到。此外，SPARC也存在于软结缔组织中。尽管SPP-1的分布比SPARC更局限，但除了成骨细胞外，肥大软骨细胞、骨膜细胞和骨髓间隙中的一些基质细胞也被染色。BSP、SPARC和SPP-1在骨和矿化软骨中的分布及细胞表达差异表明，这些蛋白在矿化结缔组织的形成和重塑中发挥着不同的功能。
