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使用L-赖氨酸高分子量聚合物作为载玻片涂层改善免疫细胞化学的切片黏附。

Improved section adhesion for immunocytochemistry using high molecular weight polymers of L-lysine as a slide coating.

作者信息

Huang W M, Gibson S J, Facer P, Gu J, Polak J M

出版信息

Histochemistry. 1983;77(2):275-9. doi: 10.1007/BF00506570.

DOI:10.1007/BF00506570
PMID:6188729
Abstract

Poly-L-lysine (PPL) has been used to coat glass slides in the preparation of tissue sections for immunocytochemical staining. The adhesive properties of different molecular weight (m.w.) polymers of L-lysine have been tested on pre-fixed cryostat sections which were subjected to a 3 day washing treatment. It has been found that the higher the molecular weight of the polymer, the greater the adhesive force it provides. PLL (m.w. 350,000) at concentrations in the range of 0.05-0.1% was found to be the most effective polymer.

摘要

聚-L-赖氨酸(PPL)已被用于在制备用于免疫细胞化学染色的组织切片时包被载玻片。对不同分子量(m.w.)的L-赖氨酸聚合物的粘附特性进行了测试,这些聚合物作用于经过3天洗涤处理的预固定低温恒温器切片。已发现聚合物的分子量越高,其提供的粘附力就越大。发现浓度在0.05 - 0.1%范围内的PLL(m.w. 350,000)是最有效的聚合物。

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J Clin Pathol. 1980 Mar;33(3):309-11. doi: 10.1136/jcp.33.3.309.
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Histochem J. 1983 May;15(5):475-82. doi: 10.1007/BF01002701.
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