金担子素A的结构

Structure of aureobasidin A.

作者信息

Ikai K, Takesako K, Shiomi K, Moriguchi M, Umeda Y, Yamamoto J, Kato I, Naganawa H

机构信息

Biotechnology Research Laboratories, Takara Shuzo Co., Ltd., Shiga, Japan.

出版信息

J Antibiot (Tokyo). 1991 Sep;44(9):925-33. doi: 10.7164/antibiotics.44.925.

DOI:10.7164/antibiotics.44.925

PMID:1938614

Abstract

Aureobasidin A, a new antifungal antibiotic, was isolated from the culture medium of Aureobasidium pullulans R106. Aureobasidin A was a cyclic depsipeptide consisting of eight alpha-amino acid units and one hydroxy acid unit. The structures of the units were found by acid hydrolysis of the antibiotic to be 2(R)-hydroxy-3(R)-methylpentanoic acid, beta-hydroxy-N-methyl-L-valine, N-methyl-L-valine, L-proline, allo-L-isoleucine, N-methyl-L-phenylalanine, L-leucine, and L-phenyl-alanine. The sequence of the units was identified by NMR and FAB-MS of the products from the alkaline hydrolysis of aureobasidin A.

摘要

新型抗真菌抗生素金担子素A是从出芽短梗霉R106的培养基中分离得到的。金担子素A是一种环缩肽，由八个α-氨基酸单元和一个羟基酸单元组成。通过对抗生素进行酸水解发现，这些单元的结构分别为2(R)-羟基-3(R)-甲基戊酸、β-羟基-N-甲基-L-缬氨酸、N-甲基-L-缬氨酸、L-脯氨酸、别-L-异亮氨酸、N-甲基-L-苯丙氨酸、L-亮氨酸和L-苯丙氨酸。通过对金担子素A碱性水解产物进行核磁共振（NMR）和快原子轰击质谱（FAB-MS）分析，确定了这些单元的序列。