[肺癌患者与炎性渗出液患者的壳三糖苷酶、溶菌酶和组织蛋白酶H的血清活性（初步报告）]

Gołab Krzysztof, Passowicz-Muszyńska Ewa, Jankowska Renata, Warwas Maria

Department of Pharmaceutical Biochemistry, Medical University of Wroclaw, Poland.

Pol Merkur Lekarski. 2009 Mar;26(153):194-7.

UNLABELLED

Lung cancer remains the leading cause of cancer death over the world. Although new diagnostic methods have been discovered, new biomarkers of the cancer are still under studying. A human chitinolytic enzyme called chitotriosidase hydrolyzes chitin and chitotrioside substrates. It is specifically expressed by activating macrophages and seems to play a role in the defense against chitinous human pathogens. Recently it has been shown that chitotriosidase may also attend to the inflammatory process. The aim of the study was to determine chitotriosidase activity in serum of patients with lung cancer and patients with inflammatory exudate. We studied the usefulness of the above parameter determination in differentiation between lung cancer and inflammation. In addition, serum activity of lysozyme and cathepsin H was determined.

MATERIALS AND METHODS

The study included 17 patients with inflammatory pleural exudate--group 1., 40 lung cancer patients with malignant pleural effusion--group 2. and 37 healthy subjects. All the patients of group 2. were divided into 2 subgroups: 2A without metastases (n = 23) and 2B with metastases (n = 17). Chitotriosidase and cathepsin H activity was determined in serum by a fluorometric methods. Serum lysozyme activity was measured by turbidimetric method with Micrococcus luteus as substrate.

RESULTS

We observed an increase of the chitotriosidase activity in serum patients of both studied group in comparison with the control. The activity of the chitotriosidase in lung cancer patients was significantly higher than in the control (36.7 vs 68.1 nmol/ml/h; p < 0.01). There were no significant differences in serum lysozyme and cathepsin H activity in patients in comparison to healthy subjects.

CONCLUSION

The results suggest that activity of the chitotriosidase can not be used to differentiation between inflammation and cancer in lung.

未标注

肺癌仍是全球癌症死亡的主要原因。尽管已发现新的诊断方法，但该癌症的新生物标志物仍在研究中。一种名为几丁质酶的人类几丁质分解酶可水解几丁质和几丁三糖苷底物。它由活化的巨噬细胞特异性表达，似乎在抵御含几丁质的人类病原体方面发挥作用。最近有研究表明，几丁质酶可能也参与炎症过程。本研究的目的是测定肺癌患者和炎性渗出液患者血清中的几丁质酶活性。我们研究了上述参数测定在肺癌与炎症鉴别诊断中的实用性。此外，还测定了溶菌酶和组织蛋白酶H的血清活性。

材料与方法

本研究纳入17例炎性胸腔积液患者（第1组）、40例伴有恶性胸腔积液的肺癌患者（第2组）和37名健康受试者。第2组的所有患者又分为2个亚组：无转移的2A亚组（n = 23）和有转移的2B亚组（n = 17）。采用荧光法测定血清中的几丁质酶和组织蛋白酶H活性。以藤黄微球菌为底物，采用比浊法测定血清溶菌酶活性。