Hou Hongliang, Zhang Xiaoling, Tang Tingting, Dai Kerong, Ge Ruowen
Department of Orthopaedics, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China.
Biotechnol Lett. 2009 Aug;31(8):1183-9. doi: 10.1007/s10529-009-0007-4. Epub 2009 Apr 24.
To explore the potential of combined delivery of osteogenic and angiogenic factors to bone marrow stromal cells (BMSCs) for repair of critical-size bone defects, we followed the formation of bone and vessels in tissue-engineered constructs in nude mice and rabbit bone defects upon introducing different combinations of BMP-2, vascular endothelial growth factor (VEGF) and angiopoietin-1 (Ang-1) to BMSCs with adenoviral vectors. Better osteogenesis and angiogenesis were found in co-delivery group of BMP-2, VEGF and angiopoietin-1 than any other combination of these factors in both animal models, indicating combined gene delivery of angiopoietin-1 and VEGF165 into a tissue-engineered construct produces an additive effect on BMP-2-induced osteogenesis.
为了探索联合递送成骨因子和血管生成因子至骨髓基质细胞（BMSCs）以修复临界尺寸骨缺损的潜力，我们通过腺病毒载体将骨形态发生蛋白-2（BMP-2）、血管内皮生长因子（VEGF）和血管生成素-1（Ang-1）的不同组合导入BMSCs，随后在裸鼠组织工程构建体以及兔骨缺损模型中追踪骨和血管的形成情况。在两种动物模型中，BMP-2、VEGF和血管生成素-1联合递送组的成骨和血管生成情况均优于这些因子的其他任何组合，这表明将血管生成素-1和VEGF165联合基因递送至组织工程构建体对BMP-2诱导的成骨具有累加效应。
