Ninth People's Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China.
Arch Oral Biol. 2010 Mar;55(3):195-202. doi: 10.1016/j.archoralbio.2010.01.006. Epub 2010 Feb 9.
To study the effects of maxillary sinus floor elevation by a tissue engineered bone complex with bone morphogenetic protein-2 (BMP-2) gene modified bone marrow stromal cells (bMSCs) and a novel porous ceramic scaffold (OsteoBone) in rabbits.
bMSCs derived from New Zealand rabbit bone marrow were cultured and transduced with adenovirus AdBMP-2 and with AdEGFP gene (without BMP-2 gene sequence) as a control, respectively, in vitro. These bMSCs were then combined with OsteoBone scaffold at a concentration of 2 x 10(7)cells/ml and used to elevate the maxillary sinus floor in rabbits. Eight rabbits were randomly allocated into groups and sacrificed at weeks 2 and 4. For each time point, 8 maxillary sinus floor elevation surgeries were made bilaterally in 4 rabbits for the two groups (n=4 per group): group A (AdBMP-2-bMSCs/material) and group B (AdEGFP-bMSCs/material). All samples were evaluated by histologic and histomorphometric analysis.
The augmented maxillary sinus height was maintained for both groups over the entire experimental period, while new bone area increased over time for group A. At week 4 after operation, bone area in group A was significantly more than that in group B (P<0.05), and was more obviously detected in the center of the elevated space. Under a confocal microscope, green fluorescence in newly formed bone was observed in the EGFP group, which suggests that those implanted bMSCs had contributed to the new bone formation.
bMSCs modified with AdBMP-2 gene can promote new bone formation in elevating the rabbit maxillary sinus. OsteoBone scaffold could be an ideal carrier for gene enhanced bone tissue engineering.
研究经腺病毒 AdBMP-2 基因修饰的骨髓基质细胞（bMSCs）和新型多孔陶瓷支架（OsteoBone）复合组织工程骨复合物提升兔上颌窦底的效果。
体外培养新西兰兔骨髓来源的 bMSCs，分别用腺病毒 AdBMP-2 和 AdEGFP 基因（无 BMP-2 基因序列）转染，作为对照。将这些 bMSCs 与 OsteoBone 支架以 2×10(7)细胞/ml 的浓度混合，用于提升兔上颌窦底。8 只兔随机分为两组，分别于第 2、4 周处死。对于每个时间点，在 4 只兔的双侧进行 8 次上颌窦底提升手术，用于两组（每组 n=4）：A 组（AdBMP-2-bMSCs/材料）和 B 组（AdEGFP-bMSCs/材料）。所有样本均进行组织学和组织形态计量学分析。
两组上颌窦提升高度在整个实验期间均得到维持，而 A 组新骨面积随时间增加。术后 4 周，A 组骨面积明显大于 B 组（P<0.05），且在提升空间的中心更为明显。在共聚焦显微镜下，可观察到 EGFP 组新形成的骨中有绿色荧光，表明植入的 bMSCs 有助于新骨形成。
AdBMP-2 基因修饰的 bMSCs 可促进兔上颌窦提升中新骨形成。OsteoBone 支架可为基因增强骨组织工程的理想载体。
