Saedler Rainer, Jakoby Marc, Marin Birger, Galiana-Jaime Elena, Hülskamp Martin
University of Cologne, Botanical Institute, 50931 Cologne, Germany.
Plant J. 2009 Aug;59(4):612-21. doi: 10.1111/j.1365-313X.2009.03900.x. Epub 2009 Apr 25.
WD40/BEACH domain proteins have been implicated in membrane trafficking and membrane composition events in Dictyostelium and Drosophila. In this paper, we show that the Arabidopsis SPIRRIG (SPI) gene encodes a WD40/BEACH domain protein. The cellular analysis revealed fragmented vacuoles in root hairs similar to those found in the corresponding Dictyostelium mutants, suggesting a related cellular function. The phenotypic analysis revealed that spi mutants share all phenotypic aspects of mutants in the actin polymerization-regulating ARP2/3 pathway, including distorted trichomes, less lobing of epidermal pavement cells, disconnected epidermal cells on various organs, and shorter root hairs. This complete phenotypic overlap suggests that this WD40/BEACH domain protein and the actin-regulating ARP2/3 pathway are involved in similar growth processes.
WD40/BEACH结构域蛋白已被证明参与了盘基网柄菌和果蝇中的膜运输及膜组成事件。在本文中,我们表明拟南芥SPIRRIG(SPI)基因编码一种WD40/BEACH结构域蛋白。细胞分析显示根毛中的液泡碎片化,类似于在相应的盘基网柄菌突变体中发现的情况,这表明它们具有相关的细胞功能。表型分析表明,spi突变体与肌动蛋白聚合调节ARP2/3途径中的突变体具有所有表型特征,包括毛状体扭曲、表皮铺板细胞叶裂减少、各器官上的表皮细胞分离以及根毛较短。这种完全的表型重叠表明,这种WD40/BEACH结构域蛋白和肌动蛋白调节ARP2/3途径参与了相似的生长过程。