Lojzova Lenka, Riddellova Katerina, Hajslova Jana, Zrostlikova Jitka, Schurek Jakub, Cajka Tomas
Institute of Chemical Technology, Prague, Faculty of Food and Biochemical Technology, Department of Food Chemistry and Analysis, Technicka 3, 166 28 Prague 6, Czech Republic.
Anal Chim Acta. 2009 May 8;641(1-2):101-9. doi: 10.1016/j.aca.2009.03.036. Epub 2009 Mar 31.
Several methods have been developed for the analysis of substituted pyrazines and related substances in potato chips. Following separation/detection approaches (all employing head-space solid phase microextraction, HS-SPME, for volatiles sampling), have been critically assessed in our study: (i) gas chromatography-ion trap mass spectrometry (GC-ITMS), (ii) gas chromatography-time-of-flight mass spectrometry (GC-TOFMS); (iii) comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC x GC-TOFMS). Although in none of the tested systems full chromatographic resolution of some isomeric pairs could be achieved, the use of GC x GC-TOFMS offered the best solution, mainly because of distinctly lower limits of quantification (LOQs) for all of 13 target alkylpyrazines. In addition to good performance characteristics, a non-target screening and tentative identification of altogether 46 N-containing heterocyclic compounds (pyrazines, pyrrols, pyridines, pyrrolidinones, and tetrahydropyridines) was also enabled.
已经开发出几种分析薯片中亚取代吡嗪及相关物质的方法。在我们的研究中，对以下分离/检测方法（所有方法均采用顶空固相微萃取（HS-SPME）进行挥发性物质采样）进行了严格评估：（i）气相色谱-离子阱质谱法（GC-ITMS），（ii）气相色谱-飞行时间质谱法（GC-TOFMS）；（iii）全二维气相色谱-飞行时间质谱法（GC×GC-TOFMS）。尽管在所有测试系统中均无法实现某些异构体对的完全色谱分离，但GC×GC-TOFMS的使用提供了最佳解决方案，主要是因为13种目标烷基吡嗪的定量下限（LOQ）明显更低。除了具有良好的性能特征外，还能够对总共46种含氮杂环化合物（吡嗪、吡咯、吡啶、吡咯烷酮和四氢吡啶）进行非目标筛选和初步鉴定。
