Jolivalt Corinne G, Mizisin Leah M, Nelson Austin, Cunha Joice M, Ramos Khara M, Bonke Dieter, Calcutt Nigel A
Department of Pathology, University of California, San Diego, USA.
Eur J Pharmacol. 2009 Jun 10;612(1-3):41-7. doi: 10.1016/j.ejphar.2009.04.028. Epub 2009 Apr 23.
There are sporadic reports that assorted combinations of B vitamins can alleviate pain in diabetic patients, but there is neither agreement on the relative efficacy of individual B vitamins nor understanding of the mechanisms involved. We therefore investigated the efficacy of a cocktail of the vitamins B1, B6 and B12 in alleviating behavioral indices of sensory dysfunction such as allodynia and hyperalgesia in diabetic rats and also the relative contribution of individual components of the cocktail. Repeated daily treatment with the cocktail of B vitamins for 7-9 days ameliorated tactile allodynia and formalin-evoked hyperalgesia in a dose-dependent manner and also improved sensory nerve conduction velocity in diabetic rats. Investigation of the contribution of individual B vitamins suggested that all three participated with variable efficacy in the alleviation of allodynia after protracted, but not single dose treatment. Only vitamin B6 improved sensory nerve conduction velocity slowing in diabetic rats when given alone. To address potential mechanisms of action, we measured markers of oxidative stress (lipid and protein oxidation) and inflammation (cyclooxygenase-2 (COX-2) and TNFalpha protein) in the nerve but treatment with the vitamin B cocktail did not significantly affect any of these parameters. The positive effects of B vitamins on functional and behavioral disorders of diabetic rats suggest a potential for use in treating painful diabetic neuropathy.
有零星报道称，多种组合的B族维生素可缓解糖尿病患者的疼痛，但对于单一B族维生素的相对疗效以及所涉及的机制尚无定论。因此，我们研究了维生素B1、B6和B12的组合在减轻糖尿病大鼠感觉功能障碍的行为指标（如异常性疼痛和痛觉过敏）方面的疗效，以及该组合中各成分的相对作用。每天重复给予B族维生素组合治疗7 - 9天，可剂量依赖性地改善糖尿病大鼠的触觉异常性疼痛和福尔马林诱发的痛觉过敏，并提高感觉神经传导速度。对单一B族维生素作用的研究表明，在长期（而非单次剂量）治疗后，所有三种维生素都以不同的效力参与了异常性疼痛的缓解。单独给予时，只有维生素B6改善了糖尿病大鼠感觉神经传导速度的减慢。为了探究潜在的作用机制，我们测量了神经中的氧化应激标志物（脂质和蛋白质氧化）和炎症标志物（环氧化酶 - 2（COX - 2）和TNFα蛋白），但B族维生素组合治疗并未显著影响这些参数中的任何一个。B族维生素对糖尿病大鼠功能和行为障碍具有积极作用，提示其在治疗疼痛性糖尿病神经病变方面具有潜在应用价值。
