Gajdus Jerzy, Kaczyński Zbigniew, Smietana Joanna, Stepnowski Piotr
Faculty of Chemistry, University of Gdańsk, Gdańsk, Poland.
Carbohydr Res. 2009 May 26;344(8):1054-7. doi: 10.1016/j.carres.2009.02.015. Epub 2009 Feb 25.
The O-antigenic polysaccharide of Salmonella Mara O:39 (formerly Q) was investigated by sugar and methylation analyses, absolute configuration assignment, mass spectrometry and NMR spectroscopy. The experiments revealed an O-polysaccharide chain composed of the following linear tetrasaccharide repeating units with the structure: -->2)-alpha-L-Quip3NAc-(1-->3)-alpha-D-Manp-(1-->3)-alpha-L-Fucp-(1-->3)-alpha-D-GalpNAc-(1--> where alpha-L-Quip3NAc is the residue of 3-acetamido-3,6-dideoxy-alpha-L-glucopyranose. This repeating unit is the first published structure of the O-polysaccharide from 27 serotypes of Salmonella bacteria belonging to serogroup O:39 in the Kauffmann-White classification system.
通过糖分析、甲基化分析、绝对构型确定、质谱分析和核磁共振光谱分析，对鼠伤寒沙门氏菌O:39（原Q）的O抗原多糖进行了研究。实验揭示了一种由以下线性四糖重复单元组成的O多糖链，其结构为：→2)-α-L-喹糖胺-3-乙酸酯-(1→3)-α-D-甘露糖-(1→3)-α-L-岩藻糖-(1→3)-α-D-氨基半乳糖-(1→，其中α-L-喹糖胺-3-乙酸酯是3-乙酰氨基-3,6-二脱氧-α-L-吡喃葡萄糖的残基。这个重复单元是考夫曼-怀特分类系统中属于O:39血清群的27种沙门氏菌血清型的O多糖的首个公开结构。
