Coulam Carolyn B, Roussev Roumen G, Lerner Sveltana, Zlatopolsky Zev, Ilkevitch Yuri, Tur-Kaspa Ilan
Rinehart Center for Reproductive Medicine, Evanston, Illinois, USA.
Fertil Steril. 2009 Jun;91(6):2408-13. doi: 10.1016/j.fertnstert.2008.03.081. Epub 2009 Apr 25.
To determine if soluble human leukocyte antigen-G (sHLA-G) concentrations in spent culture media may assist in identifying the normal embryo for implantation.
Prospective blinded comparative study.
Reproductive genetic and reproductive medicine centers.
PATIENT(S): One hundred and sixteen embryos obtained from eight patients undergoing in vitro fertilization (IVF) with preimplantation genetic diagnosis (PGD).
INTERVENTION(S): Culture media obtained 2 days after fertilization were analyzed for sHLA-G concentrations using an enzyme-linked immunosorbent assay (ELISA) assay. A sHLA-G concentration of >or=1.9 mIU/mL was considered a positive predictor for successful implantation. Polar bodies and blastomeres from day-3 embryos were tested by PGD for 5 to 11 chromosomes: 8, 9, 13, 15, 16, 17, 18, 21, 22, X, and Y.
MAIN OUTCOME MEASURE(S): The results of the sHLA-G concentrations were compared with the results of the PGD analyses.
RESULT(S): We found an sHLA-G concentration >or=1.9 mIU/mL in 48% (56 out of 116) and normal PGD results in 52% (57 out of 116) of embryos. Of the embryos with normal PGD results, 46% (26 out of 57) had sHLA-G concentrations >or=1.9 mIU/mL. Among the embryos with sHLA-G >or=1.9 mIU/mL, 46% (26 out of 56) had normal PGD results, and 21% of embryos displayed both normal PGD results and sHLA-G >or=1.9 mIU/mL.
CONCLUSION(S): No correlation between concentrations of sHLA-G in embryo culture media and PGD results of an embryo's aneuploidy were observed.
确定废弃培养基中可溶性人类白细胞抗原-G（sHLA-G）浓度是否有助于识别正常可植入胚胎。
前瞻性盲法对照研究。
生殖遗传学和生殖医学中心。
从8例接受体外受精（IVF）并进行植入前基因诊断（PGD）的患者中获取116个胚胎。
受精2天后获取的培养基，采用酶联免疫吸附测定（ELISA）法分析sHLA-G浓度。sHLA-G浓度≥1.9 mIU/mL被视为成功植入的阳性预测指标。对第3天胚胎的极体和卵裂球进行PGD检测5至11条染色体：8、9、13、15、16、17、18、21、22、X和Y。
将sHLA-G浓度结果与PGD分析结果进行比较。
我们发现116个胚胎中有48%（56个）的sHLA-G浓度≥1.9 mIU/mL，52%（57个）的胚胎PGD结果正常。在PGD结果正常的胚胎中，46%（26个）的sHLA-G浓度≥1.9 mIU/mL。在sHLA-G≥1.9 mIU/mL的胚胎中，46%（26个）的PGD结果正常，21%的胚胎PGD结果正常且sHLA-G≥1.9 mIU/mL。
未观察到胚胎培养基中sHLA-G浓度与胚胎非整倍体的PGD结果之间存在相关性。
