Sher Geoffrey, Keskintepe Levent, Fisch Jeffery D, Acacio Brian A, Ahlering Peter, Batzofin Joel, Ginsburg Mike
Sher Institutes for Reproductive Medicine, New York, New York 10016, USA.
Fertil Steril. 2005 May;83(5):1410-3. doi: 10.1016/j.fertnstert.2004.11.061.
To compare pregnancy and implantation rates after ART when embryos for day 3 embryo transfer were selected based on soluble HLA-G (sHLA-G) expression in the culture media at 46 hours after fertilization by intracytoplasmic sperm injection (ICSI).
Prospective cohort study.
Private practice.
PATIENT(S): One hundred seven patients undergoing ART aged <39 years with normal ovarian reserve, a normal uterine cavity, and two or more embryos scoring > or =70 by the graduated embryo scoring (GES) method, transferred on day 3.
INTERVENTION(S): Patients were divided into two groups. In group A (n = 51) all embryos transferred expressed sHLA-G above the geometric mean (sHLA-G+), whereas in group B (n = 56) all embryos transferred were sHLA-G-ve.
MAIN OUTCOME MEASURE(S): Viable pregnancy rate (patients with fetal heart activity at 8 weeks of gestation per embryo transfer procedure), and implantation rate (viable gestational sacs per total embryos transferred).
RESULT(S): When all embryos transferred were sHLA-G+ve the pregnancy and implantation rates were 75% (38/51) and 44% (51/116), respectively, compared to 23% (13/56) and 14% (20/143) when all embryos transferred were sHLA-G-ve.
CONCLUSION(S): Pregnancy and implantation rates after day 3 embryo transfer are improved when sHLA-G expression in phase I culture media at 46 hours after fertilization by ICSI is used prospectively as a criterion for selecting optimal embryos for transfer.
比较在卵胞浆内单精子注射（ICSI）受精后46小时，根据培养基中可溶性人类白细胞抗原G（sHLA-G）表达情况选择第3天胚胎进行移植时，辅助生殖技术（ART）后的妊娠率和着床率。
前瞻性队列研究。
私人诊所。
107例接受ART的患者，年龄<39岁，卵巢储备正常，子宫腔正常，且有两个或更多胚胎通过分级胚胎评分（GES）法评分≥70分，于第3天进行移植。
患者分为两组。A组（n = 51）所有移植胚胎的sHLA-G表达高于几何平均数（sHLA-G+），而B组（n = 56）所有移植胚胎的sHLA-G为阴性（sHLA-G-ve）。
活产妊娠率（每次胚胎移植手术在妊娠8周时出现胎心活动的患者）和着床率（移植的全部胚胎中存活的妊娠囊数）。
当所有移植胚胎的sHLA-G为阳性时，妊娠率和着床率分别为75%（38/51）和44%（51/116），而当所有移植胚胎的sHLA-G为阴性时，妊娠率和着床率分别为23%（13/56）和14%（20/143）。
当将ICSI受精后46小时I期培养基中的sHLA-G表达情况前瞻性地用作选择最佳移植胚胎的标准时，第3天胚胎移植后的妊娠率和着床率会提高。
