Murray S, Wellings R, Taylor I K, Fuller R W, Taylor G W
Department of Clinical Pharmacology, Royal Postgraduate Medical School, London, U.K.
J Chromatogr. 1991 Jul 5;567(2):289-98.
An assay has been developed for N tau-methylhistamine, a major metabolite of the autocoid histamine, based on gas chromatography-electron-capture negative-ion chemical ionisation mass spectrometry. N tau-Methylhistamine was extracted from urine by cation-exchange chromatography and converted to its di-(3,5-bistrifluoromethylbenzoyl) derivative. The latter has good chromatographic properties and gives a negative-ion mass spectrum with the molecular ion (M., m/z 605) as base peak. A commercially available trideuterated analogue of N tau-methylhistamine was used as internal standard. Basal urinary excretion of N tau-methylhistamine in five normal subjects was found to be 0.21 +/- 0.05 mumol/h (289 +/- 74 mumol/mol of creatinine). This value was not significantly altered in these subjects following the infusion of a subpharmacological dose of histamine. In eight atopic volunteers, basal urinary excretion of N tau-methylhistamine was also not significantly changed following challenge with inhaled allergen.
基于气相色谱 - 电子捕获负离子化学电离质谱法，已开发出一种针对自泌素组胺的主要代谢产物Nτ - 甲基组胺的检测方法。通过阳离子交换色谱法从尿液中提取Nτ - 甲基组胺，并将其转化为其二 -（3,5 - 双三氟甲基苯甲酰）衍生物。后者具有良好的色谱特性，给出的负离子质谱以分子离子（M.，m/z 605）作为基峰。使用市售的Nτ - 甲基组胺三氘代类似物作为内标。发现五名正常受试者中Nτ - 甲基组胺的基础尿排泄量为0.21±0.05μmol/h（289±74μmol/mol肌酐）。在这些受试者中，输注亚药理剂量的组胺后，该值没有显著改变。在八名特应性志愿者中，吸入过敏原激发后，Nτ - 甲基组胺的基础尿排泄量也没有显著变化。
