Oosting E, Keyzer J J
ABL Analytical Biochemical Laboratory, Assen, The Netherlands.
Agents Actions. 1991 May;33(1-2):215-7. doi: 10.1007/BF01993171.
A newly developed radioimmunoassay (RIA, Y) for the determination of urinary N tau-methylhistamine concentrations was correlated with gas chromatography mass spectrometry (GCMS, X). In 34 urine samples, with histamine and N tau-methylhistamine levels within our reference values, the correlation was: Y = 1.47X -0.245 mumol/l (r = 0.92; p-slope less than or equal to 0.0001). In 14 pathological urine samples, derived from patients with mastocytosis and having upper reference values, the correlation was: Y = 1.75X - 1.02 mumol/l (r = 0.93; p-slope less than or equal to 0.001). In spite of the greater specificity of the monoclonal antibody for N tau-methylhistamine compared with that of histamine, relatively high urinary histamine concentrations gave a false positive influence on the RIA results, which was 100% when the histamine/N tau-methylhistamine ratio was about 19. Clear cases of mastocytosis can be diagnosed, using the RIA-kit, but for a more precise N tau-methylhistamine value GCMS analyses will remain necessary.
一种新开发的用于测定尿中N-τ-甲基组胺浓度的放射免疫分析方法（RIA，Y）与气相色谱-质谱联用技术（GCMS，X）进行了相关性研究。在34份组胺和N-τ-甲基组胺水平在我们参考值范围内的尿样中，相关性为：Y = 1.47X - 0.245 μmol/L（r = 0.92；p斜率≤0.0001）。在14份来自肥大细胞增多症患者且组胺和N-τ-甲基组胺水平高于参考值的病理性尿样中，相关性为：Y = 1.75X - 1.02 μmol/L（r = 0.93；p斜率≤0.001）。尽管与组胺相比，针对N-τ-甲基组胺的单克隆抗体具有更高的特异性，但尿中相对较高的组胺浓度对放射免疫分析结果产生了假阳性影响，当组胺/N-τ-甲基组胺比值约为19时，假阳性率为100%。使用放射免疫分析试剂盒可以诊断明确的肥大细胞增多症病例，但要获得更精确的N-τ-甲基组胺值，气相色谱-质谱联用分析仍然是必要的。
