Kesik Vural, Guven Ahmet, Vurucu Sabahattin, Tunc Turan, Uysal Bulent, Gundogdu Gokhan, Oztas Emin, Korkmaz Ahmet
Department of Pediatrics, Gulhane Military Medical Academy, Etlik, Ankara, Turkey.
J Surg Res. 2009 Nov;157(1):e97-e105. doi: 10.1016/j.jss.2008.12.024. Epub 2009 Jan 14.
Acute intestinal ischemia reperfusion (I/R) injury affects not only the intestines but also remote organs due to pro-inflammatory and tissue injurious factors. Thus, we aimed to investigate the roles of melatonin (a powerful antioxidant) and 1400W (a strong inhibitor of inducible nitric oxide) in a rat intestinal I/R injury model, since oxidative and nitrosative injury are believed to be the major causes.
A total of 56 Wistar albino rats were used, with seven rats in each group. After I/R induction in the intestines by clamping/unclamping the superior mesenteric artery, we measured malondialdehyde, superoxide dismutase, glutathione peroxidase, nitric oxide, and 3-nitrotyrosine levels in lung, kidney, and liver tissues (to evaluate remote organ injury) as well as in the intestines. Study groups received melatonin, 1400W or both to examine the roles of these molecules in the pathogenesis of injury following I/R.
Melatonin and 1400W had an ameliorating effect on both oxidative and nitrosative stress in the intestine and the lung against mesenteric I/R injury in rats. Moreover, each of these two agents had an inhibitory effect on oxidative injury and histopathological changes in the intestine and the lung. Furthermore, the combination of both agents (melatonin and 1400W) was more effective than either of the agents alone (P < 0.05).
Melatonin and 1400W, either alone or in combination, were efficient in ameliorating experimental I/R injury of the intestines.
急性肠缺血再灌注（I/R）损伤不仅会影响肠道，还会因促炎和组织损伤因子而影响远处器官。因此，我们旨在研究褪黑素（一种强大的抗氧化剂）和1400W（一种强力诱导型一氧化氮抑制剂）在大鼠肠I/R损伤模型中的作用，因为氧化和亚硝化损伤被认为是主要原因。
总共使用56只Wistar白化大鼠，每组7只。通过夹闭/松开肠系膜上动脉诱导肠道I/R后，我们测量了肺、肾和肝组织（以评估远处器官损伤）以及肠道中的丙二醛、超氧化物歧化酶、谷胱甘肽过氧化物酶、一氧化氮和3-硝基酪氨酸水平。研究组接受褪黑素、1400W或两者，以研究这些分子在I/R后损伤发病机制中的作用。
褪黑素和1400W对大鼠肠系膜I/R损伤后的肠道和肺中的氧化和亚硝化应激均有改善作用。此外，这两种药物中的每一种对肠道和肺中的氧化损伤和组织病理学变化都有抑制作用。此外，两种药物（褪黑素和1400W）联合使用比单独使用任何一种药物更有效（P<0.05）。
褪黑素和1400W单独或联合使用均能有效改善实验性肠I/R损伤。
