Kasicka V, Prusík Z
Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, Prague.
J Chromatogr. 1991 Sep 13;569(1-2):123-74. doi: 10.1016/0378-4347(91)80228-5.
This paper gives a broad and detailed review of the applications of one of the modern high-performance electromigration separation techniques--capillary isotachophoresis (ITP)--in peptide analysis. Examples are presented of the utilization of capillary ITP for peptide analysis in the fields of chemistry, general and clinical biochemistry, biology, biotechnology, pharmacy and the food industry. The complete composition of all the electrolyte systems used for peptide ITP analyses in both cationic and anionic techniques is given in tabular form. According to the purpose of analysis the applications are divided into several sections: model studies, determination of physico-chemical characteristics, purity control of both intermediate and final peptide preparations, including the determination of low-molecular-mass ionogenic admixtures, and the analysis of peptides in biological fluids and tissue extracts. In addition to the main applications the theoretical and methodological aspects of peptide ITP analysis are discussed. The basic electromigration properties of peptides (their polyampholyte character, effective and absolute mobilities, acid-base equilibria) are explained and the selection of parameters for peptide ITP analysis is described in detail. The advantages and disadvantages of ITP compared with other electrophoretic and chromatographic methods used for peptide analysis are discussed.
本文对现代高效电迁移分离技术之一——毛细管等速电泳（ITP）在肽分析中的应用进行了广泛而详细的综述。文中列举了毛细管ITP在化学、普通和临床生物化学、生物学、生物技术、制药及食品工业等领域用于肽分析的实例。以表格形式给出了在阳离子和阴离子技术中用于肽ITP分析的所有电解质系统的完整组成。根据分析目的，应用分为几个部分：模型研究、物理化学特性的测定、中间肽制剂和最终肽制剂的纯度控制（包括低分子质量离子性杂质的测定）以及生物体液和组织提取物中肽的分析。除了主要应用外，还讨论了肽ITP分析的理论和方法学方面。解释了肽的基本电迁移特性（其聚两性电解质特性、有效迁移率和绝对迁移率、酸碱平衡），并详细描述了肽ITP分析参数的选择。讨论了ITP与用于肽分析的其他电泳和色谱方法相比的优缺点。
