Cupo J F
BASF Bioresearch Corporation, Cambridge, MA 02139.
J Chromatogr. 1991 Sep 13;569(1-2):389-406. doi: 10.1016/0378-4347(91)80239-9.
Nuclear matrix proteins form the skeleton of the nucleus and participate in the various cellular functions of the nucleus. These proteins have been demonstrated to be tissue-type specific and can potentially reflect changes in the state of differentiation of the cell. Elucidating nuclear matrix protein changes necessitates the use of high-resolution two-dimensional polyacrylamide gel electrophoresis. Separation of this complex mixture into its component parts resolves protein changes when comparing the normal state to a diseased state of a cell. Evidence has been reviewed which shows the potential use of nuclear matrix proteins and antibodies to nuclear matrix proteins as diagnostic tools for various cancers, autoimmune diseases, adenoviral infection, and other diseases. Consequently, the central functions of the nuclear matrix in the cell allow it to have significant potential as a diagnostic agent.
核基质蛋白构成细胞核的骨架，并参与细胞核的各种细胞功能。这些蛋白质已被证明具有组织类型特异性，并可能反映细胞分化状态的变化。阐明核基质蛋白的变化需要使用高分辨率二维聚丙烯酰胺凝胶电泳。将这种复杂混合物分离成其组成部分，可以在比较细胞的正常状态和患病状态时解析蛋白质的变化。已有证据表明，核基质蛋白和核基质蛋白抗体作为各种癌症、自身免疫性疾病、腺病毒感染和其他疾病的诊断工具具有潜在用途。因此，核基质在细胞中的核心功能使其具有作为诊断剂的巨大潜力。
