Getzenberg R H, Pienta K J, Huang E Y, Coffey D S
Department of Urology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Cancer Res. 1991 Dec 15;51(24):6514-20.
The nuclear matrix is the structural component of the nucleus that determines nuclear morphology and organizes the DNA in a three-dimensional fashion that is tissue specific. Previously, some of the nuclear matrix proteins have been reported to be both tissue and cell type specific and are altered with the state of differentiation and transformation. This study demonstrates that the nuclear matrix is specific for the individual lobes of the normal rat prostate and that the nuclear matrix undergoes changes in protein composition in the Dunning prostate cancer tissue. Additionally, in the Dunning rat prostate adenocarcinoma cell lines, there is a range of tumor phenotypes and the nuclear matrix varies in composition in each tumor cell type. These differences in the nuclear matrix proteins are associated with quantitative changes in nuclear morphology that form the pleiomorphic state of the cancer nucleus.
核基质是细胞核的结构成分，它决定细胞核形态，并以三维方式组织DNA，这种方式具有组织特异性。此前，已有报道称一些核基质蛋白具有组织和细胞类型特异性，并随分化和转化状态而改变。本研究表明，核基质对正常大鼠前列腺的各个叶具有特异性，并且在邓宁前列腺癌组织中核基质的蛋白质组成会发生变化。此外，在邓宁大鼠前列腺腺癌细胞系中，存在一系列肿瘤表型，并且每种肿瘤细胞类型的核基质组成各不相同。核基质蛋白的这些差异与细胞核形态的定量变化相关，这些变化形成了癌细胞核的多形状态。
