Siegel A, Marota J J, Boas D
Opt Express. 1999 Apr 12;4(8):287-98. doi: 10.1364/oe.4.000287.
Diffuse optical tomography (DOT) can image spatial variations in highly scattering optical media. We have built an inexpensive and portable continuous-wave DOT system containing 18 laser diode sources (9 at 780nm and 9 at 830nm) and 16 silicon detectors, which can acquire 288 independent measurements in less than 4 seconds. These data can then be processed using a variety of imaging algorithms. We first discuss the design of diffuse imaging equipment in general, and then describe our instrument, along with the technical issues that influenced its design. The technical challenges involved in performing DOT over large optode areas are discussed. We also present rat brain measurements following electrical forepaw stimulation using DOT. These results clearly demonstrate the capabilities of DOT and set the stage for advancement to quantitative functional brain imaging.
扩散光学层析成像（DOT）能够对高散射光学介质中的空间变化进行成像。我们构建了一个价格低廉且便携的连续波DOT系统，该系统包含18个激光二极管光源（9个波长为780nm，9个波长为830nm）和16个硅探测器，能够在不到4秒的时间内获取288个独立测量值。然后可以使用各种成像算法对这些数据进行处理。我们首先总体讨论扩散成像设备的设计，接着描述我们的仪器以及影响其设计的技术问题。讨论了在大光极区域进行DOT所涉及的技术挑战。我们还展示了使用DOT对大鼠前爪电刺激后的脑部测量结果。这些结果清楚地证明了DOT的能力，并为推进到定量功能性脑成像奠定了基础。
