Truncated Newton's optimization scheme for absorption and fluorescence optical tomography: Part II Reconstruction from synthetic measurements.

Using two dimensional synthetic frequency-domain measurements, the inverse imaging problem is solved for absorption and fluorescence lifetime mapping with the truncated Newtons optimization scheme developed in Part I of this contribution. Herein, we present reconstructed maps of absorption owing to a fluorophore from excitation and emission measurements which detail the presence of tissue heterogeneities characterized by tenfold increase in fluorescent contrast agent. Our results confirm that fluorescence provides superior mapping of heterogeneities over excitation measurements. Using emission measurements we then map fluorescent lifetime under conditions of tenfold uptake of contrast agent in tissue heterogeneities. The ability to map fluorescent quenching and lengthening of contrast agents facilitates the solution of the inverse problem and further improves the ability to reconstruct tissue heterogeneities.