III型分泌装置细胞外部分的结构剖析
Structural dissection of the extracellular moieties of the type III secretion apparatus.
作者信息
Wang Yu, Zhang Lingling, Picking Wendy L, Picking William D, De Guzman Roberto N
机构信息
Department of Molecular Biosciences, The University of Kansas, Lawrence, KS 66045, USA.
出版信息
Mol Biosyst. 2008 Dec;4(12):1176-80. doi: 10.1039/b808271p. Epub 2008 Sep 18.
Abstract
Many Gram-negative bacterial pathogens use type III secretion systems (TTSSs) for subverting the normal cellular functions of their target eukaryotic cells. The type III secretion apparatus (TTSA) functions like a syringe to inject proteins through an external needle and into a target cell's membrane and cytosol. The TTSA basal body spans the bacterial inner and outer membranes, and the external needle is topped with a tip complex that controls the secretion and delivery of translocator and effector proteins. Recently solved structures of TTSA proteins have greatly advanced our understanding of shared themes in apparatus assembly and function. In this highlight, the structure-function of TTSA needle and tip complex proteins are described and common themes discussed.
摘要
许多革兰氏阴性细菌病原体利用III型分泌系统（TTSSs）来颠覆其靶真核细胞的正常细胞功能。III型分泌装置（TTSA）的功能就像一个注射器，通过外部针头将蛋白质注射到靶细胞的膜和细胞质中。TTSA基体跨越细菌的内膜和外膜，外部针头顶部有一个尖端复合体，该复合体控制转运蛋白和效应蛋白的分泌与递送。最近解析的TTSA蛋白结构极大地推动了我们对该装置组装和功能中共同主题的理解。在本综述中，描述了TTSA针头和尖端复合体蛋白的结构功能，并讨论了共同主题。
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