Rathert Philipp, Dhayalan Arunkumar, Ma Huimin, Jeltsch Albert
Biochemistry Laboratory, School of Engineering and Science, Jacobs University Bremen, Campus Ring 1, 28759 Bremen, Germany.
Mol Biosyst. 2008 Dec;4(12):1186-90. doi: 10.1039/b811673c. Epub 2008 Oct 20.
Post translational modification of histone proteins including lysine methylation is an important epigenetic mark, essential for gene regulation and development. Recently, several examples of lysine methylation of non-histone proteins have been discovered suggesting that this is a common post-translational modification for regulation of protein activity. Here, we review assays for the detection of protein methylation based on mass spectrometry, radiolabel and immunological approaches using protein and peptide substrates including application of SPOT peptide arrays. Candidates for new methylation targets of protein methyltransferases can be predicted using the specificity of the enzyme and protein interaction data.
包括赖氨酸甲基化在内的组蛋白的翻译后修饰是一种重要的表观遗传标记，对基因调控和发育至关重要。最近，已发现非组蛋白赖氨酸甲基化的几个实例，这表明这是调节蛋白质活性的常见翻译后修饰。在这里，我们综述了基于质谱、放射性标记和免疫方法检测蛋白质甲基化的分析方法，这些方法使用蛋白质和肽底物，包括SPOT肽阵列的应用。可以利用酶的特异性和蛋白质相互作用数据预测蛋白质甲基转移酶新甲基化靶点的候选物。
