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解码蛋白质甲基化组：鉴定、验证及功能见解

Decoding the protein methylome: Identification, validation, and functional insights.

作者信息

Meng Ying, Huang Rong

机构信息

Borch Department of Medicinal Chemistry and Molecular Pharmacology, Purdue Institute for Drug Discovery, Purdue University Center for Cancer Research, Purdue University, West Lafayette, IN 47907, United States.

Borch Department of Medicinal Chemistry and Molecular Pharmacology, Purdue Institute for Drug Discovery, Purdue University Center for Cancer Research, Purdue University, West Lafayette, IN 47907, United States.

出版信息

Bioorg Med Chem. 2025 Feb 1;118:118056. doi: 10.1016/j.bmc.2024.118056. Epub 2024 Dec 28.

DOI:10.1016/j.bmc.2024.118056
PMID:39754853
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11735303/
Abstract

Protein methylation regulates diverse cellular processes including gene expression and DNA repair. This review discusses the methods of identifying and validating substrates for protein methyltransferases (MTases), as well as the biological roles of methylation. Meanwhile, we outline continued efforts necessary to fully map MTase-substrate pairs and uncover the complex regulatory roles of protein methylation in cellular function.

摘要

蛋白质甲基化调节多种细胞过程，包括基因表达和DNA修复。本综述讨论了鉴定和验证蛋白质甲基转移酶（MTases）底物的方法，以及甲基化的生物学作用。同时，我们概述了全面绘制MTase-底物对并揭示蛋白质甲基化在细胞功能中的复杂调节作用所需的持续努力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4287/11735303/53bc34bc4aa1/nihms-2046066-f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4287/11735303/f3b06c55587c/nihms-2046066-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4287/11735303/b605fbb1fe2e/nihms-2046066-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4287/11735303/cfa9faae35b4/nihms-2046066-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4287/11735303/ec70d6aa4eca/nihms-2046066-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4287/11735303/53bc34bc4aa1/nihms-2046066-f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4287/11735303/f3b06c55587c/nihms-2046066-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4287/11735303/b605fbb1fe2e/nihms-2046066-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4287/11735303/cfa9faae35b4/nihms-2046066-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4287/11735303/ec70d6aa4eca/nihms-2046066-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4287/11735303/53bc34bc4aa1/nihms-2046066-f0006.jpg

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本文引用的文献

1
Characterizations of Protein Arginine Deiminase 1 as a Substrate of NTMT1: Implications of Nα-Methylation in Protein Stability and Interaction.蛋白精氨酸脱亚氨酶 1 作为 NTMT1 底物的特性研究：Nα-甲基化对蛋白质稳定性和相互作用的影响。
J Proteome Res. 2024 Oct 4;23(10):4589-4600. doi: 10.1021/acs.jproteome.4c00484. Epub 2024 Sep 17.
2
Hydrophobic Derivatization Strategy Facilitates Comprehensive Profiling of Protein Methylation.疏水衍生化策略促进蛋白质甲基化的全面分析。
J Proteome Res. 2023 Oct 6;22(10):3275-3281. doi: 10.1021/acs.jproteome.3c00318. Epub 2023 Sep 22.
3
Identification of nonhistone substrates of the lysine methyltransferase PRDM9.
鉴定赖氨酸甲基转移酶 PRDM9 的非组蛋白底物。
J Biol Chem. 2023 May;299(5):104651. doi: 10.1016/j.jbc.2023.104651. Epub 2023 Mar 25.
4
Global lysine methylome profiling using systematically characterized affinity reagents.基于系统鉴定的亲和试剂进行全局赖氨酸甲基化组谱分析。
Sci Rep. 2023 Jan 7;13(1):377. doi: 10.1038/s41598-022-27175-x.
5
Arginine methylation of BRD4 by PRMT2/4 governs transcription and DNA repair.PRMT2/4 介导的 BRD4 精氨酸甲基化调控转录和 DNA 修复。
Sci Adv. 2022 Dec 9;8(49):eadd8928. doi: 10.1126/sciadv.add8928. Epub 2022 Dec 7.
6
Analogs of -Adenosyl--Methionine in Studies of Methyltransferases.甲基转移酶研究中S-腺苷-L-甲硫氨酸的类似物
Mol Biol. 2022;56(2):229-250. doi: 10.1134/S002689332202011X. Epub 2022 Apr 14.
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SETD2 Restricts Prostate Cancer Metastasis by Integrating EZH2 and AMPK Signaling Pathways.SETD2 通过整合 EZH2 和 AMPK 信号通路来限制前列腺癌转移。
Cancer Cell. 2020 Sep 14;38(3):350-365.e7. doi: 10.1016/j.ccell.2020.05.022. Epub 2020 Jul 2.
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Proteome-wide identification of arginine methylation in colorectal cancer tissues from patients.对患者结直肠癌组织中精氨酸甲基化进行全蛋白质组鉴定。
Proteome Sci. 2020 May 19;18:6. doi: 10.1186/s12953-020-00162-8. eCollection 2020.
9
Comparative proteomic analysis of the brain and colon in three rat models of irritable bowel syndrome.三种肠易激综合征大鼠模型中脑与结肠的蛋白质组学比较分析
Proteome Sci. 2020 Feb 24;18:1. doi: 10.1186/s12953-020-0157-9. eCollection 2020.
10
methylation of OLA1 revealed by activity-based target profiling of NTMT1.通过NTMT1的基于活性的靶点分析揭示的OLA1甲基化。
Chem Sci. 2019 Aug 9;10(35):8094-8099. doi: 10.1039/c9sc02550b. eCollection 2019 Sep 21.