环介导等温扩增技术（LAMP）：一种用于传染病的快速、准确且经济高效的诊断方法。
Loop-mediated isothermal amplification (LAMP): a rapid, accurate, and cost-effective diagnostic method for infectious diseases.
作者信息
Mori Yasuyoshi, Notomi Tsugunori
机构信息
Biochemical Research Laboratory, Research and Development Division, Eiken Chemical Co., Ltd., 1381-3 Shimoishigami, Ohtawara, Tochigi, 324-0036, Japan.
出版信息
J Infect Chemother. 2009 Apr;15(2):62-9. doi: 10.1007/s10156-009-0669-9. Epub 2009 Apr 25.
Abstract
Loop-mediated isothermal amplification (LAMP) is an established nucleic acid amplification method offering rapid, accurate, and cost-effective diagnosis of infectious diseases. This technology has been developed into commercially available detection kits for a variety of pathogens including bacteria and viruses. The current focus on LAMP methodology is as a diagnostic system to be employed in resource-limited laboratories in developing countries, where many fatal tropical diseases are endemic. The combination of LAMP and novel microfluidic technologies such as Lab-on-a-chip may facilitate the realization of genetic point-of-care testing systems to be used by both developed and developing countries in the near future. This review will describe the historical, current, and future developments of such technologies.
摘要
环介导等温扩增技术（LAMP）是一种成熟的核酸扩增方法，可快速、准确且经济高效地诊断传染病。该技术已开发成针对包括细菌和病毒在内的多种病原体的商用检测试剂盒。目前，LAMP方法的重点是作为一种诊断系统，供发展中国家资源有限的实验室使用，这些国家流行许多致命的热带疾病。LAMP与新型微流控技术（如芯片实验室）相结合，可能会促进在不久的将来发达国家和发展中国家都能使用的即时基因检测系统的实现。本文综述将描述此类技术的历史、现状和未来发展。
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