通过结合基于核酸序列的扩增和逆转录环介导等温扩增检测的两步等温扩增检测系统快速灵敏地检测牡蛎中的诺如病毒基因组
Rapid and sensitive detection of norovirus genomes in oysters by a two-step isothermal amplification assay system combining nucleic acid sequence-based amplification and reverse transcription-loop-mediated isothermal amplification assays.
作者信息
Fukuda Shinji, Sasaki Yukie, Seno Masato
机构信息
Center for Public Health and Environment, Hiroshima Prefectural Technology Research Institute, Minami-machi 1-6-29, Minami-ku, Hiroshima 734-0007, Japan.
出版信息
Appl Environ Microbiol. 2008 Jun;74(12):3912-4. doi: 10.1128/AEM.00127-08. Epub 2008 May 2.
Abstract
We developed a two-step isothermal amplification assay system, which achieved the detection of norovirus (NoV) genomes in oysters with a sensitivity similar to that of reverse transcription-seminested PCR. The time taken for the amplification of NoV genomes from RNA extracts was shortened to about 3 h.
摘要
我们开发了一种两步等温扩增检测系统，该系统能够检测牡蛎中的诺如病毒（NoV）基因组，其灵敏度与逆转录半巢式PCR相似。从RNA提取物中扩增NoV基因组所需的时间缩短至约3小时。
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