Kierzek Elzbieta, Christensen Shawn M, Eickbush Thomas H, Kierzek Ryszard, Turner Douglas H, Moss Walter N
Department of Chemistry, University of Rochester, NY 14627-0216, USA.
J Mol Biol. 2009 Jul 17;390(3):428-42. doi: 10.1016/j.jmb.2009.04.048. Epub 2009 May 3.
Sequences from the 5' region of R2 retrotransposons of four species of silk moth are reported. In Bombyx mori, this region of the R2 messenger RNA contains a binding site for R2 protein and mediates interactions critical to R2 element insertion into the host genome. A model of secondary structure for a segment of this RNA is proposed on the basis of binding to oligonucleotide microarrays, chemical mapping, and comparative sequence analysis. Five conserved secondary structures are identified, including a novel pseudoknot. There is an apparent transition from an entirely RNA structure coding function in most of the 5' segment to a protein coding function near the 3' end. This suggests that local regions evolved under separate functional constraints (structural, coding, or both).
报道了四种蚕蛾R2反转录转座子5'区域的序列。在家蚕中，R2信使RNA的该区域包含R2蛋白的结合位点，并介导R2元件插入宿主基因组所必需的相互作用。基于与寡核苷酸微阵列的结合、化学图谱分析和比较序列分析，提出了该RNA一段序列的二级结构模型。鉴定出五个保守的二级结构，包括一个新的假结。在5'端大部分区域存在从完全编码功能的RNA结构到靠近3'端蛋白质编码功能的明显转变。这表明局部区域是在不同的功能限制（结构、编码或两者兼有）下进化的。
