Andrade Pablo, Fernández-Guasti Alonso, Carrillo-Ruiz José D, Ulloa Rosa E, Ramírez Ylián, Reyes Rebeca, Jiménez Fiacro
Functional Neurosurgery, Stereotaxy and Radiosurgery Unit, Mexico General Hospital, and Anáhuac University, School of Medicine and Psychology, Mexico City, Mexico.
Behav Brain Res. 2009 Oct 12;203(1):108-12. doi: 10.1016/j.bbr.2009.04.026. Epub 2009 May 3.
Stereotaxic lesions of the orbitofrontal-thalamic system, specifically the thalamic reticular nucleus (TRN), could be helpful to prevent perseverative behavior in rats produced by 8-OH-DPAT.
Fifty rats were conditioned in a T-maze to measure the number of perseverations. Habituation was performed on days 1 and 2, baseline scores were obtained on day 3 and the final test was done on day 4 (chemical induction). Group I only received saline solution injection; group II was only submitted to 8-OH-DPAT; group III received pharmacological treatment with chlorimipramine (CMI) before 8-OH-DPAT administration; group IV and group V were submitted to stereotaxic bilateral lesions, one week before T-maze evaluation, in the TRN and orbitofrontal cortex (OFC), respectively and received 8-OH-DPAT administration.
No differences between groups were found at baseline on day 3 (p<0.05). Significant differences were found between days 3 and 4 of evaluation only in group II (p<0.01) and group V (p<0.001). Differences between groups on day 4 were significant (p<0.01).
TRN lesions were as effective as CMI administration to prevent the 8-OH-DPAT action. OFC lesions failed to prevent the perseverative behavior.
眶额-丘脑系统的立体定向损伤，特别是丘脑网状核（TRN），可能有助于预防由8-羟基二苯丙胺（8-OH-DPAT）诱发的大鼠持续性行为。
50只大鼠在T型迷宫中进行条件训练以测量持续性行为的次数。第1天和第2天进行习惯化训练，第3天获得基线分数，第4天进行最终测试（化学诱导）。第一组仅注射生理盐水；第二组仅注射8-OH-DPAT；第三组在给予8-OH-DPAT之前接受氯米帕明（CMI）药物治疗；第四组和第五组分别在T型迷宫评估前一周，对TRN和眶额皮质（OFC）进行立体定向双侧损伤，并给予8-OH-DPAT。
第3天基线时各组之间无差异（p<0.05）。仅在第二组（p<0.01）和第五组（p<0.001）的评估第3天和第4天之间发现显著差异。第4天各组之间的差异显著（p<0.01）。
TRN损伤在预防8-OH-DPAT作用方面与给予CMI同样有效。OFC损伤未能预防持续性行为。
