Cho Minjung, Cho Wan-Seob, Choi Mina, Kim Sueng Jun, Han Beom Seok, Kim Sheen Hee, Kim Hyoung Ook, Sheen Yhun Yhong, Jeong Jayoung
Division of Toxicologic Pathology, National Institute of Toxicological Research, Korea Food and Drug Administration, 231 Jinhongno Enpyung-ku, Seoul 122-704, Republic of Korea.
Toxicol Lett. 2009 Sep 28;189(3):177-83. doi: 10.1016/j.toxlet.2009.04.017. Epub 2009 May 3.
Many approaches for the application of nano-sized particles to the human body as nanotechnology have been recently developed. The size of nanoparticles is related to their useful character and also plays a key role in toxicity. Since this surface area can interact with biological components of cells, nanoparticles can be more reactive in than larger particles. In the present study, a fluorescence dye-labeled 50, 100 and 200 nm-sized silica particle suspension was intravenously injected into mice to identify the toxicity, tissue distribution and excretion of silica nanoparticles in vivo. Incidence and severity of inflammatory response was transiently increased with injection of 200 and 100 nm silica nanoparticles within 12h. But there was no significant response related to injection of 50 nm particles. The silica particles of 50, 100 and 200 nm were cleared via urine and bile. The 50 nm silica nanoparticles cleared to urine and bile than 100 nm and particles of 200 nm existed at lower concentration than other two smaller particles in urine and feces. Silica nanoparticles were trapped by macrophages in the spleen and liver and remained there until 4 weeks after the single injection.
作为纳米技术，近期已开发出许多将纳米颗粒应用于人体的方法。纳米颗粒的尺寸与其有用特性相关，并且在毒性方面也起着关键作用。由于这种表面积可与细胞的生物成分相互作用，纳米颗粒可能比更大的颗粒更具反应性。在本研究中，将荧光染料标记的50、100和200纳米大小的二氧化硅颗粒悬浮液静脉注射到小鼠体内，以确定二氧化硅纳米颗粒在体内的毒性、组织分布和排泄情况。在12小时内注射200纳米和100纳米二氧化硅纳米颗粒后，炎症反应的发生率和严重程度短暂增加。但注射50纳米颗粒未出现明显反应。50、100和200纳米的二氧化硅颗粒通过尿液和胆汁清除。50纳米二氧化硅纳米颗粒比100纳米的清除到尿液和胆汁中的速度更快，并且在尿液和粪便中，200纳米颗粒的浓度低于其他两种较小颗粒。二氧化硅纳米颗粒被脾脏和肝脏中的巨噬细胞捕获，并在单次注射后4周内一直留在那里。
