Yamaguchi T, Handa K, Shimizu Y, Abo T, Kumagai K
J Immunol. 1977 Jun;118(6):1931-5.
Whole leukocytes, mononuclear cells, polymorphonuclear cells (PMN), MONOCYTES, PURIFIED LYMPHOCYTES, AND T (rosette-forming cells, RFC) and non-T (nonrosette-forming cells, nonRFC) lymphocytes isolated from the human peripheral blood were stimulated by Sendai virus, respectively, and examined for interferon production in their culture fluids. High levels of interferon were produced by mononuclear cells, but not by PMN. Removal of monocytes from the mononuclear cell population did not affect at all the levels of interferon produced, although it strongly suppressed interferon induction by polyinosinic-polycytidylic acid (poly IC) and mitogenic response to phytohemagglutinin (PHA) of the lymphocytes. Purified monocytes and T lymphocytes were unresponsive to the virus. In contrast, a population of purified non-T lymphocytes produced high levels of interferon. Addition of monocytes to the interferon-producing non-T lymphocytes did not affect the levels of interferon produced. No detectable levels of interferon were produced in the mixture of T lymphocytes and monocytes. It is concluded that non-T lymphocytes may be a major target for interferon induction of human leukocytes by Sendai virus.
从人外周血中分离出的全白细胞、单核细胞、多形核细胞（PMN）、单核细胞、纯化淋巴细胞以及T（形成玫瑰花结细胞，RFC）和非T（不形成玫瑰花结细胞，nonRFC）淋巴细胞，分别用仙台病毒刺激，并检测其培养液中的干扰素产生情况。单核细胞产生高水平的干扰素，而PMN则不产生。从单核细胞群体中去除单核细胞对产生的干扰素水平完全没有影响，尽管它强烈抑制了多聚肌苷酸-多聚胞苷酸（poly IC）诱导的干扰素以及淋巴细胞对植物血凝素（PHA）的促有丝分裂反应。纯化的单核细胞和T淋巴细胞对该病毒无反应。相反，一群纯化的非T淋巴细胞产生高水平的干扰素。向产生干扰素的非T淋巴细胞中添加单核细胞并不影响产生的干扰素水平。在T淋巴细胞和单核细胞的混合物中未检测到干扰素水平。结论是，非T淋巴细胞可能是仙台病毒诱导人白细胞产生干扰素的主要靶细胞。
