Davis Joel, Yu Daozhan, Evans Wendy, Gokirmak Tufan, Chetelat Roger T, Stotz Henrik U
Department of Horticulture, Oregon State University, Corvallis, OR 97331, USA.
Theor Appl Genet. 2009 Jul;119(2):305-14. doi: 10.1007/s00122-009-1039-9. Epub 2009 Apr 28.
Cultivated tomato (Solanum lycopersicum, syn. Lycopersicon esculentum) is susceptible to the necrotrophic ascomycete and causal agent of gray mold, Botrytis cinerea. Resistance to this fungal pathogen is elevated in wild relatives of tomato, including Solanum lycopersicoides. An introgression line population (IL) containing chromosomal segments of S. lycopersicoides within the background of tomato cv. VF36 was used to screen the genome for foliar resistance and susceptibility to B. cinerea. Based on this screen, putative quantitative trait loci (QTL) were identified, five for resistance and two for susceptibility. Four resistance QTL decreased infection frequency while the fifth reduced lesion diameter. One susceptibility QTL increased infection frequency whereas the other increased lesion diameter. Overlapping chromosomal segments provided strong evidence for partial resistance on chromosomes 1 and 9 and for elevated susceptibility on chromosome 11. Segregation analysis confirmed the major resistance QTL on the long arm of chromosome 1 and susceptibility on chromosome 11. Linkage of partial resistance to chromosome 9 could not be confirmed. The usefulness of these data for resistance breeding and for map-based cloning of foliar resistance to B. cinerea is discussed.
栽培番茄（Solanum lycopersicum，同物异名Lycopersicon esculentum）对坏死营养型子囊菌及灰霉病的病原菌灰葡萄孢（Botrytis cinerea）敏感。番茄的野生近缘种，包括多毛番茄（Solanum lycopersicoides），对这种真菌病原体的抗性有所提高。一个渗入系群体（IL）在番茄品种VF36的背景中含有多毛番茄的染色体片段，用于筛选基因组对灰葡萄孢叶部抗性和敏感性。基于此筛选，鉴定出了假定的数量性状位点（QTL），其中五个与抗性相关，两个与敏感性相关。四个抗性QTL降低了感染频率，而第五个降低了病斑直径。一个敏感性QTL增加了感染频率，而另一个增加了病斑直径。重叠的染色体片段为第1和第9号染色体上的部分抗性以及第11号染色体上的易感性增加提供了有力证据。分离分析证实了第1号染色体长臂上的主要抗性QTL和第11号染色体上的敏感性。与第9号染色体部分抗性的连锁关系未能得到证实。讨论了这些数据在抗性育种以及基于图谱克隆对灰葡萄孢叶部抗性方面的用途。
