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利用杂交作物中的克隆配子构建多倍体基因组。

Harnessing clonal gametes in hybrid crops to engineer polyploid genomes.

作者信息

Wang Yazhong, Fuentes Roven Rommel, van Rengs Willem M J, Effgen Sieglinde, Zaidan Mohd Waznul Adly Mohd, Franzen Rainer, Susanto Tamara, Fernandes Joiselle Blanche, Mercier Raphael, Underwood Charles J

机构信息

Department of Chromosome Biology, Max Planck Institute for Plant Breeding Research, Cologne, Germany.

Central Microscopy (CeMic), Max Planck Institute for Plant Breeding Research, Cologne, Germany.

出版信息

Nat Genet. 2024 Jun;56(6):1075-1079. doi: 10.1038/s41588-024-01750-6. Epub 2024 May 13.

DOI:10.1038/s41588-024-01750-6
PMID:38741016
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11176054/
Abstract

Heterosis boosts crop yield; however, harnessing additional progressive heterosis in polyploids is challenging for breeders. We bioengineered a 'mitosis instead of meiosis' (MiMe) system that generates unreduced, clonal gametes in three hybrid tomato genotypes and used it to establish polyploid genome design. Through the hybridization of MiMe hybrids, we generated '4-haplotype' plants that encompassed the complete genetics of their four inbred grandparents, providing a blueprint for exploiting polyploidy in crops.

摘要

杂种优势可提高作物产量；然而，对于育种者来说，在多倍体中利用额外的渐进杂种优势具有挑战性。我们通过生物工程构建了一个“有丝分裂而非减数分裂”（MiMe）系统，该系统在三种杂交番茄基因型中产生未减数的克隆配子，并利用它来建立多倍体基因组设计。通过MiMe杂种的杂交，我们培育出了“4-单倍型”植株，这些植株包含了其四个自交系祖父母的完整遗传信息，为在作物中利用多倍体提供了蓝图。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fef1/11176054/3652958397f2/41588_2024_1750_Fig4_ESM.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fef1/11176054/0c6915553bee/41588_2024_1750_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fef1/11176054/4b36c90b0ea3/41588_2024_1750_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fef1/11176054/72193faaa745/41588_2024_1750_Fig3_ESM.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fef1/11176054/3652958397f2/41588_2024_1750_Fig4_ESM.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fef1/11176054/0c6915553bee/41588_2024_1750_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fef1/11176054/4b36c90b0ea3/41588_2024_1750_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fef1/11176054/72193faaa745/41588_2024_1750_Fig3_ESM.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fef1/11176054/3652958397f2/41588_2024_1750_Fig4_ESM.jpg

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