Sun Yi, Kwok Yien-Chian, Foo-Peng Lee Peter, Nguyen Nam-Trung
National Institute of Education, Nanyang Technological University, 1 Nanyang Walk, Singapore, 637616, Singapore.
Anal Bioanal Chem. 2009 Jul;394(5):1505-8. doi: 10.1007/s00216-009-2808-7. Epub 2009 Apr 28.
The use of genetically modified organisms (GMOs) as food and in food products is becoming more and more widespread. Polymerase chain reaction (PCR) technology is extensively used for the detection of GMOs in food products in order to verify compliance with labeling requirements. In this paper, we present a novel close-loop ferrofluid-driven PCR microchip for rapid amplification of GMOs. The microchip was fabricated in polymethyl methacrylate by CO2 laser ablation and was integrated with three temperature zones. PCR solution was contained in a circular closed microchannel and was driven by magnetic force generated by an external magnet through a small oil-based ferrofluid plug. Successful amplification of genetically modified soya and maize were achieved in less than 13 min. This PCR microchip combines advantages of cycling flexibility and quick temperature transitions associated with two existing microchip PCR techniques, and it provides a cost saving and less time-consuming way to conduct preliminary screening of GMOs.
转基因生物（GMOs）在食品及食品生产中的应用正变得越来越广泛。聚合酶链反应（PCR）技术被广泛用于食品中转基因生物的检测，以验证是否符合标签要求。在本文中，我们展示了一种新型的闭环铁磁流体驱动的PCR微芯片，用于快速扩增转基因生物。该微芯片由聚甲基丙烯酸甲酯通过二氧化碳激光烧蚀制成，并集成了三个温度区。PCR溶液包含在一个圆形封闭微通道中，并由外部磁铁通过一个小的油基铁磁流体塞产生的磁力驱动。在不到13分钟的时间内成功扩增了转基因大豆和玉米。这种PCR微芯片结合了循环灵活性和与两种现有微芯片PCR技术相关的快速温度转变的优点，并且它为转基因生物的初步筛选提供了一种节省成本且耗时较少的方法。
