Department of General Surgery, Xiangya Second Hospital, Xiangya Medical College, Central South University, Changsha, Hunan, China.
Med Oncol. 2010 Jun;27(2):339-45. doi: 10.1007/s12032-009-9215-y. Epub 2009 Apr 28.
Golgi protein-73 (GP73) is a newly identified candidate serum marker for HCC, but GP73 study now is lesser in Asian population. The aims of this study were to determine how GP73 is detected in the serum of healthy, hepatitis B, cirrhosis and HCC by western blotting and RT-PCR, and to establish the sensitivity and specificity of serum GP73 protein and RNA for diagnosing HCC. Serum GP73 was detected by western blotting and RT-PCR, and quantified by densitometric analysis. GP73 was measured in serum from 124 patients with various forms of liver. AFP was tested using commercially available electrochemiluminescence immunoassay. The median sGP73 in patients with HBV-related HCC was significantly higher (P < 0.001) than in healthy individuals and in patients with other diseases. When sGP73 protein was used to detect HBV-related HCC, it had a sensitivity of 77.4% and a specificity of 83.9%, at the optimal cut-off value of 7.4 relative units. The area under the receiver-operating characteristic curve was 0.89. GP73 RNA in patients with HBV-related HCC had a sensitivity of 87.1% and a specificity of 83.9% and AUROC of 0.92. AFP in patients with HCC had a sensitivity of 48.4% and a specificity of 96.8% and AUROC of 0.77. GP73 protein and RNA can be found in the serum of patients with HBV-related HCC obviously higher than of other liver diseases in Asian. GP73 was better than AFP for the diagnosis of HBV-related HCC. RT-PCR is a more sensitive and superior method of quantification than Western blot. Furthermore, our data need to be confirmed in larger cohorts of patients.
高尔基蛋白 73（GP73）是一种新发现的 HCC 血清标志物候选物，但目前亚洲人群对 GP73 的研究较少。本研究旨在通过 Western blot 和 RT-PCR 确定 GP73 在健康人群、乙型肝炎、肝硬化和 HCC 患者血清中的检测情况，并建立血清 GP73 蛋白和 RNA 诊断 HCC 的敏感性和特异性。通过 Western blot 和 RT-PCR 检测血清 GP73，并通过密度计分析进行定量。检测了 124 例不同类型肝病患者的血清 GP73。使用市售电化学发光免疫分析法检测 AFP。HBV 相关 HCC 患者的中位 sGP73 明显高于（P < 0.001）健康个体和其他疾病患者。当 sGP73 蛋白用于检测 HBV 相关 HCC 时，其最佳截断值为 7.4 相对单位时，敏感性为 77.4%，特异性为 83.9%。ROC 曲线下面积为 0.89。HBV 相关 HCC 患者的 GP73 RNA 敏感性为 87.1%，特异性为 83.9%，ROC 曲线下面积为 0.92。HCC 患者 AFP 的敏感性为 48.4%，特异性为 96.8%，ROC 曲线下面积为 0.77。GP73 蛋白和 RNA 可在 HBV 相关 HCC 患者的血清中明显高于其他肝病患者。GP73 比 AFP 更适合诊断 HBV 相关 HCC。RT-PCR 是一种比 Western blot 更敏感和优越的定量方法。此外，我们的数据需要在更大的患者队列中得到验证。
