Navarrete Gómez P, Espinoza Ruiz J, Parodi Rivera J, Alvarez J G, Sánchez Gutiérrez R
Centro de Excelencia de Biotecnología de la Reproducción (CEBIOR), Universidad de La Frontera, Temuco, Chile.
Andrologia. 2009 Jun;41(3):196-8. doi: 10.1111/j.1439-0272.2009.00922.x.
The integrity of the paternal genome is of paramount importance in the initiation and maintenance of a viable pregnancy. Oxygen radicals (ROS) have been identified as one of the main factors responsible for the induction of sperm DNA damage. Spermatozoa are mainly protected against ROS-induced damage by seminal plasma. However, this protective effect disappears once spermatozoa enter the female genital tract. The fallopian tube mucosa may play a protective role against ROS-induced sperm damage. The main objective of this study was to determine whether human tubal explants and tubal fluid exert a protective effect on ROS-induced sperm DNA damage. Spermatozoa were exposed to tubal explants and/or tubal fluid in the presence of phorbol myristate acetate (PMA)-activated polymorphonuclear leucocytes or control medium and sperm DNA fragmentation was measured using the TdT-mediated dUTP-biotin nick end labelling (TUNEL) test. Exposure of human spermatozoa to PMA-activated leucocytes resulted in a 2-fold increase in sperm DNA fragmentation. Co-incubation of spermatozoa with tubal explants did not reduce this damage. However, pre-incubation of spermatozoa with tubal fluid resulted in a statistically significant reduction in sperm DNA fragmentation levels, comparable to those observed in control. In conclusion, tubal fluid appears to protect against activated leucocyte-induced sperm DNA fragmentation, thus preserving the integrity of the paternal genome.
父本基因组的完整性对于成功受孕的起始和维持至关重要。氧自由基（ROS）已被确定为导致精子DNA损伤的主要因素之一。精子主要受到精浆的保护，免受ROS诱导的损伤。然而，一旦精子进入女性生殖道，这种保护作用就会消失。输卵管黏膜可能对ROS诱导的精子损伤起到保护作用。本研究的主要目的是确定人输卵管外植体和输卵管液是否对ROS诱导的精子DNA损伤具有保护作用。在佛波酯肉豆蔻酸酯乙酸酯（PMA）激活的多形核白细胞或对照培养基存在的情况下，将精子暴露于输卵管外植体和/或输卵管液中，并使用TdT介导的dUTP生物素缺口末端标记（TUNEL）试验测量精子DNA片段化。将人精子暴露于PMA激活的白细胞会导致精子DNA片段化增加2倍。精子与输卵管外植体共同孵育并未减少这种损伤。然而，精子与输卵管液预孵育导致精子DNA片段化水平在统计学上显著降低，与对照组观察到的水平相当。总之，输卵管液似乎可以保护精子免受激活的白细胞诱导的DNA片段化，从而保持父本基因组的完整性。
