Brzozowski T, Majka J, Garlicki J, Drozdowicz D, Konturek S J
Institute of Physiology, Academy of Medicine, Krakow, Poland.
J Clin Gastroenterol. 1991;13 Suppl 1:S98-102. doi: 10.1097/00004836-199112001-00016.
Epidermal growth factor (EGF) exhibits its gastroprotective action against a variety of irritants and ulcerogens and plays an important role in healing of acute and chronic gastroduodenal ulcerations, but the mechanisms of these effects are not known. The present study was undertaken to determine whether polyamines (such as spermine or putrescine) and prostaglandins (PGs), which also show protective properties, contribute to the gastroprotective effect of EGF against ethanol injury in rats. It was found that both EGF and polyamines significantly and dose-dependently prevented the formation of gastric lesions induced by absolute ethanol, the effect being similar to that obtained with 16,16-dimethylprostaglandin E2. Pretreatment with indomethacin failed to affect the gastroprotective action of EGF and polyamines, suggesting that endogenous PGs may not play any major role in this protection. Our finding that the protective effect of EGF can be abolished by pretreatment with DFMO, an inhibitor of the polyamine biosynthetic pathway, suggests that gastroprotection by EGF is due, at least in part, to stimulation of biosynthesis of protective polyamines.
表皮生长因子（EGF）对多种刺激物和致溃疡剂具有胃保护作用，在急慢性胃十二指肠溃疡的愈合中发挥重要作用，但其作用机制尚不清楚。本研究旨在确定同样具有保护特性的多胺（如精胺或腐胺）和前列腺素（PGs）是否有助于EGF对大鼠乙醇损伤的胃保护作用。结果发现，EGF和多胺均能显著且剂量依赖性地预防无水乙醇诱导的胃损伤形成，其效果与16,16-二甲基前列腺素E2相似。用吲哚美辛预处理未能影响EGF和多胺的胃保护作用，提示内源性PGs可能在这种保护中不起主要作用。我们的研究发现，多胺生物合成途径抑制剂DFMO预处理可消除EGF的保护作用，这表明EGF的胃保护作用至少部分归因于对保护性多胺生物合成的刺激。
