Abe S, Sasano H, Katoh K, Ohara S, Arikawa T, Noguchi T, Asaki S, Yasui W, Tahara E, Nagura H, Toyota T
Department of Medicine (III), Tohoku University School of Medicine, Sendai, Japan.
Dig Dis Sci. 1997 Jun;42(6):1199-209. doi: 10.1023/a:1018897922644.
Expression of members of the epidermal growth factor family, including epidermal growth factor (EGF), transforming growth factor-alpha (TGF-alpha), amphiregulin (AR), and Cripto, as well as their putative receptor, epidermal growth factor receptor (EGFR), was studied immunohistochemically in human gastric mucosa to evaluate their possible roles in cell proliferation of normal and regenerative gastric mucosa. We also examined the correlation betwen cell proliferation and EGFR by double immunohistochemical staining for proliferating cell nuclear antigen (PCNA) and EGFR. In normal gastric mucosa, TGF-alpha, Cripto, and AR immunoreactivities were observed in the surface epithelial and parietal cells of gastric fundic glands, respectively. EGF immunoreactivity was not observed in any of normal mucosa examined. EGFR immunoreactivity was detected on foveolar cells in proliferative zones and in parietal cells. Double immunostaining revealed that EGFR immunoreactivity was distributed much more widely than PCNA immunoreactivity. PCNA positive epithelial cells adjacent to gastric ulcer margin expressed relatively intense EGFR but did not express any of the growth factors examined. On the other hand, relatively intense immunoreactivity of both TGF-alpha and Cripto was detected in PCNA-negative regenerative epithelium located distant from gastric ulcer margin. Relative immunoreactivity of AR in regenerative gastric epithelium associated with ulcer was not different from that in normal gastric mucosa. TGF-alpha, AR, and Cripto are considered to play important roles in normal gastric mucosal proliferation, and TGF-alpha and Cripto may be involved in ulcer healing, possibly via a paracrine mechanism.
采用免疫组织化学方法研究了表皮生长因子家族成员，包括表皮生长因子（EGF）、转化生长因子-α（TGF-α）、双调蛋白（AR）和Cripto，以及它们的假定受体表皮生长因子受体（EGFR）在人胃黏膜中的表达情况，以评估它们在正常和再生胃黏膜细胞增殖中的可能作用。我们还通过对增殖细胞核抗原（PCNA）和EGFR进行双重免疫组织化学染色，研究了细胞增殖与EGFR之间的相关性。在正常胃黏膜中，TGF-α、Cripto和AR免疫反应性分别在胃底腺的表面上皮细胞和壁细胞中观察到。在所检查的任何正常黏膜中均未观察到EGF免疫反应性。在增殖区的小凹细胞和壁细胞中检测到EGFR免疫反应性。双重免疫染色显示，EGFR免疫反应性的分布比PCNA免疫反应性广泛得多。胃溃疡边缘相邻的PCNA阳性上皮细胞表达相对较强的EGFR，但不表达所检测的任何生长因子。另一方面，在远离胃溃疡边缘的PCNA阴性再生上皮中检测到TGF-α和Cripto的相对较强免疫反应性。与溃疡相关的再生胃上皮中AR的相对免疫反应性与正常胃黏膜中的无差异。TGF-α、AR和Cripto被认为在正常胃黏膜增殖中起重要作用，TGF-α和Cripto可能通过旁分泌机制参与溃疡愈合。
