Instituto de Virología Dr. J.M.Vanella, Universidad Nacional de Córdoba, Córdoba, Argentina.
Vector Borne Zoonotic Dis. 2010 Mar;10(2):199-201. doi: 10.1089/vbz.2008.0156.
Venezuelan Equine Encephalitis complex viruses cause epidemics and epizootics periodically in some regions of the Americas. In Argentina, only enzootic Rio Negro virus (AG80-663) (RNV) has been isolated. To survey and identify activity of viruses that belong to Venezuelan Equine Encephalitis complex in a template region of the country, a generic Alphavirus RT-Nested PCR was performed in 99 mosquito pools collected in Chaco province. Five pools were positive, and amplicons were sequenced: four of them clustered with RNV(AG80-663) and one with Pixuna virus. This is the first report of the circulation of Pixuna virus in Argentina, and it confirms enzootic and endemic activity of RNV(AG80-663) in neotropical regions of this country.
委内瑞拉马脑炎复合病毒会在美洲的一些地区定期引发流行病和动物疫情。在阿根廷，仅分离到了地方性的里尼奥内格罗病毒（AG80-663）（RNV）。为了在该国的一个模板区域内调查和识别属于委内瑞拉马脑炎复合病毒的病毒的活动情况，对在查科省采集的 99 个蚊子池中进行了通用的甲型病毒 RT-Nested PCR。有 5 个水池呈阳性，并且对扩增子进行了测序：其中 4 个与 RNV（AG80-663）聚类，1 个与皮克萨纳病毒聚类。这是首次在阿根廷报告皮克萨纳病毒的传播，证实了 RNV（AG80-663）在该国新热带地区的地方性和流行活动。
