Instituto de Virología Dr. J.M. Vanella, Facultad Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
Trop Med Int Health. 2010 Jul;15(7):865-8. doi: 10.1111/j.1365-3156.2010.02541.x. Epub 2010 May 17.
Venezuelan equine encephalitis complex includes viruses considered emerging pathogens for humans and animals in the Americas. Two members of this complex have been detected previously in Argentina: Rio Negro Virus (RNV), detected in mosquitoes from Chaco province and rodents from Formosa province, and Pixuna Virus (PIXV), detected in mosquitoes from Chaco province. To carry out surveillance studies in other parts of the country, detection of a 195-bp fragment of alphaviruses by RT-nested PCR was performed in mosquito samples from San Miguel de Tucumán city. Four pools resulted positive and three were sequenced. Two amplicons grouped with RNV and one with PIXV. This is the first report of viral activity of members of the Venezuelan equine encephalitis complex in north-eastern Argentina.
委内瑞拉马脑炎复合体包括被认为是美洲人类和动物新兴病原体的病毒。该复合体的两个成员此前在阿根廷被检测到:在查科省的蚊子和福尔摩沙省的啮齿动物中检测到里尼奥内格罗病毒(RNV),在查科省的蚊子中检测到皮克苏纳病毒(PIXV)。为了在该国其他地区开展监测研究,在圣米格尔-德图库曼市的蚊子样本中通过 RT-巢式 PCR 检测了 195bp 片段的甲病毒。四个池呈阳性,三个被测序。两个扩增子与 RNV 分组,一个与 PIXV 分组。这是在阿根廷东北部首次报告委内瑞拉马脑炎复合体成员的病毒活动。