Department of Oral and Maxillofacial Reconstructive Surgery, Okayama University Medical and Dental School, 2-5-1 Shikata, Okayama-city 7008525, Japan.
Acta Histochem. 2010 May;112(3):270-7. doi: 10.1016/j.acthis.2009.01.003. Epub 2009 Apr 29.
In the present study, we evaluated the osteogenic potential of an autogenous bone marrow graft combined with beta-tricalcium phosphate (beta-TCP) in a rat calvarial bone defect model. The bone marrow harvested from the tibia of 7-week-old rats was grafted autogenously in a calvarial defect together with beta-TCP (=BTG group, n=16) or without beta-TCP (=BG group, n=16). Groups of animals were also treated with beta-TCP alone (=TG group, n=16) and control animals (n=8) received no graft implanted into the defect. We then observed the process of bone formation by histology, enzyme histochemistry and immunohistochemistry. Five days after grafting, in the BTG and BG groups, cell proliferation and osteogenic differentiation were observed. From 5 to 10 days after surgery, active Runx2, osteopontin (OPN), and TRAP- positive cells appeared in the BTG and BG groups. New bone formation started in the defect in both the BTG and BG groups. At 30 days after grafting, the BTG group showed new bone development and replacement of beta-TCP to fill the bone defect. New bone formation in the BTG group was significantly greater than in the BG group (P<0.01). The TG group showed no marked bone formation in the defect. The combination graft of bone marrow with beta-TCP showed marked bone formation in rat calvarial defects. Our results indicate that the combination grafts of bone marrow with beta-TCP may be an effective technique for repairing bone defects Beta-TCPgraft (TG) group.
在本研究中，我们评估了自体骨髓移植与β-磷酸三钙（β-TCP）联合应用于大鼠颅骨骨缺损模型的成骨潜力。从 7 周龄大鼠胫骨中采集的骨髓与β-TCP （= BTG 组，n=16）或不与β-TCP （= BG 组，n=16）一起自体移植到颅骨缺损部位。动物还分为单独用β-TCP 治疗的 TG 组（n=16）和未植入缺陷部位的对照组（n=8）。我们通过组织学、酶组织化学和免疫组织化学观察骨形成过程。移植后 5 天，BTG 和 BG 组观察到细胞增殖和成骨分化。手术后 5-10 天，BTG 和 BG 组出现活性 Runx2、骨桥蛋白（OPN）和 TRAP 阳性细胞。BTG 和 BG 两组的缺损处均开始新骨形成。移植后 30 天，BTG 组表现出新骨发育和β-TCP 替代填充骨缺损。BTG 组的新骨形成明显大于 BG 组（P<0.01）。TG 组在缺损处无明显骨形成。骨髓与β-TCP 的联合移植在大鼠颅骨缺损处显示出明显的骨形成。我们的结果表明，骨髓与β-TCP 的联合移植可能是修复骨缺损的有效技术。β-TCP 移植（TG）组。
