Laboratoire de Chimie des Substances Naturelles et de Synthèse Organique, Faculté des Sciences de Monastir, (UR. 99/UR/12-26), 5000 Monastir, Tunisie.
Phytomedicine. 2009 Dec;16(12):1156-61. doi: 10.1016/j.phymed.2009.03.012. Epub 2009 Apr 28.
A sesquiterpenoid Bakkenolide (1), and two steroids, (3beta, 22E)-Stigmasta-5, 22-diène-3-ol (Stigmasterol) (2) and stigmasterol 3beta-glucoside (3), isolated from the Hertia cheirifolia (L.) chloroform extract, were evaluated respectively for their spasmolytic and anti-inflammatory activities. We note that these natural products were isolated and purified for the first time from the specie Hertia cheirifolia. Their structures have been established by spectroscopy (1 and 2D NMR experiences) and mass spectrometry. Chloroform-, ethyl acetate- and methanol-extracts were also tested for their spasmolytic and anti-inflammatory activities. Spasmolytic and anti-inflammatory screening were based respectively on the contractile response effects on rat isolated smooth muscles and on the dose-related carrageenan induced paw edema in rats. screening of the crude extracts showed spasmolytic and anti-inflammatory positive results. The antispasmodic effect of Bakkenolide was found in the same range as that of Alverine, a standard musculotropic spasmolytic agent.
从胡颓子科胡颓子属植物（Hertia cheirifolia）的氯仿提取物中分离得到了一种倍半萜巴卡丁内酯（1）和两种甾体化合物（3β，22E）-豆甾-5，22-二烯-3-醇（豆甾醇）（2）和豆甾醇 3β-葡萄糖苷（3）。分别对它们的平滑肌松弛和抗炎活性进行了评价。我们注意到，这些天然产物是首次从 Hertia cheirifolia 种中分离和纯化得到的。通过光谱（1 和 2D NMR 经验）和质谱确定了它们的结构。还测试了氯仿、乙酸乙酯和甲醇提取物的平滑肌松弛和抗炎活性。平滑肌松弛和抗炎筛选分别基于对大鼠分离平滑肌的收缩反应效应以及对大鼠角叉菜胶诱导的爪肿胀的剂量相关作用。粗提取物的筛选显示出平滑肌松弛和抗炎的阳性结果。巴卡丁内酯的抗痉挛作用与标准肌紧张性平滑肌松弛剂阿维林相当。
