Xu Li-Li, Han Ting, Wu Jin-Zhong, Zhang Qiao-Yan, Zhang Hong, Huang Bao-Kang, Rahman Khalid, Qin Lu-Ping
School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, PR China.
Phytomedicine. 2009 Jun;16(6-7):609-16. doi: 10.1016/j.phymed.2009.03.014. Epub 2009 Apr 28.
The chemical compositions and bioactivities of ether extracts of an endophytic fungus Paecilomyces sp. isolated from Panax ginseng were reported, and the comparative analysis of the constituents, antifungal and antitumor properties of the ether extracts from this fungus and its host ginseng were also conducted. By means of GC/MS technique, 51 compounds of Panax ginseng and 38 compounds of Paecilomyce sp. were determined. It is attractive that the extracts derived from Paecilomyce sp. and ginseng samples contained the same compound falcarinol, a natural pesticide and anti-cancer agent. The ether extracts of Paecilomyce sp., tested at 7.8 microg/ml, completely inhibited the visible growth of Pyricularia oryzae. Furthermore, both extracts were tested against four human pathogenic fungi and showed the IC(80) of Paecilomyce sp. was 4 microg/ml against Trichophyton rubrum, equally to the control. Finally, the in vitro antitumor experience showed that the most of the IC(50) values were all being below 20 microg/ml.
报道了从人参中分离得到的内生真菌拟青霉属（Paecilomyces sp.）乙醚提取物的化学成分和生物活性，并对该真菌及其宿主人参乙醚提取物的成分、抗真菌和抗肿瘤特性进行了比较分析。通过气相色谱/质谱联用（GC/MS）技术，确定了人参中的51种化合物和拟青霉属中的38种化合物。有趣的是，拟青霉属和人参样品的提取物中都含有相同的化合物法卡林二醇，一种天然杀虫剂和抗癌剂。拟青霉属的乙醚提取物在7.8微克/毫升的浓度下，完全抑制了稻瘟病菌的可见生长。此外，两种提取物都针对四种人类致病真菌进行了测试，结果显示拟青霉属对红色毛癣菌的IC(80)为4微克/毫升，与对照相当。最后，体外抗肿瘤实验表明，大多数IC(50)值均低于20微克/毫升。
